Written by an international group of eminent scientists, this new treatise is the very first in the field to provide a thorough, state-of-the-art review of the periplasm, the extracytoplasmic compartment found in gram-negative bacteria. Targeting medical practitioners and students of biology, microbiology, biochemistry, structural biology, and biotechnology, The Periplasm details important aspects of the physiology of pathogenic microorganisms, conveys a selection of current drug resistance strategies, and describes lipopolysaccharide biosynthesis.
Valuable background information outlining the inner workings of gram-negative bacteria is presented, providing insights into the evolution of cellular compartments and their benefit to living organisms. There are also comprehensive discussions of the basic biological functions of the periplasm and their physiological relevance, including protein transport, folding, and quality control; bioenergetics; solute transport; stress responses; cell division; and cell architecture. The Periplasm lays the foundation for future research directions and novel experiments in its coverage of the most up-to-date results, approaches, and hypotheses available to those working in the field. Recent insights into computational methods to predict cellular localization and the latest developments in recombinant protein production are also examined.
Section I. Protein Secretion . . 1. Co- and Post-translational Protein Targeting to the SecYEG Translocon in Escherichia coli, Eitan Bibi ... 2. The Tat Protein Export Pathway, Tracy Palmer and Ben C. Berks ... 3. Assembly of Integral Membrane Proteins from the Periplasm into the Outer Membrane, Jorg H. Kleinschmidt ... 4. Structure, Function, and Transport of Lipoproteins in Escherichia coli, Hajime Tokuda, Shin-ichi Matsuyama, and Kimie Tanaka-Masuda. Section II. Protein Folding and Quality Control. 5. The Cpx Envelope Stress Response, Tracy L. Raivio ... 6. Regulation and Function of the Envelope Stress Response Controlled by s E, Carol A. Gross, Virgil Rhodius, and Irina L. Grigorova ... 7. Disulfide Bond Formation in the Periplasm, Mehmet Berkmen, Dana Boyd, and Jon Beckwith ... 8. Periplasmic Chaperones and Peptidyl-Prolyl Isomerases, Jean-Michel Betton ... 9. Periplasmic Proteases and Protease Inhibitors, Nicolette Kucz, Michael Meltzer, and Michael Ehrmann. Section III. Key Physiological Processes. 10. Cell Division, S. J. Ryan Arends, Kyle B. Williams, Ryan J. Kustusch, and David S. Weiss ... 11. Structure and Biosynthesis of the Murein (Peptidoglycan) Sacculus, Waldemar Vollmer ... 12. Periplasmic Events in the Assembly of Bacterial Lipopolysaccharides, Chris Whitfield, Emilisa Frirdich, and Anne N. Reid ... 13. Electron Transport Activities in the Periplasm, Stuart J. Ferguson ... 14. Periplasmic Nitrate Reduction, Jeff A. Cole ... 15. Biosynthesis of the Molybdenum Cofactor and its Incorporation into Molybdoenzymes, Silke Leimkuhler ... 16. Transfer of Energy and Information across the Periplasm in Iron Transport and Regulation, Volkmar Braun and Susanne Mahren ... 17. Periplasmic ABC Transporters, Elie Dassa ... 18. Antimicrobial and Stress Resistance, Keith Poole ... 19. Osmoregulation in the Periplasm, Jean-Pierre Bohin and Jean-Marie Lacroix. Section IV. Practical Implications. 20. Practical Implications for Periplasmic Protein Accumulation, John C. Joly and Michael W. Laird ... 21. Periplasmic Expression of Antibody Fragments, David P. Humphreys. Section V. Protein Composition. 22. Methods for the Computational Prediction of Periplasmic Proteins, Jennifer L. Gardy and Fiona S. L. Brinkman
Editor: Michael Ehrmann, University of Duisburg-Essen, Germany