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Since the publication of the bestselling second edition of this title, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function.
Each readily reproducible method offers a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.
the breadth of techniques covered is large and this alone makes the book of practical value...The protocols are provided with sufficient detail so as to allow implementation as new methods in any working laboratory...It can easily be envisioned that this book would become the subject of a tug of war in a busy laboratory. Most investigators will find value here as will students working in almost any area dealing with proteins...This is a compilation not found in other single sources. The very broad scope has considerable practical value. I personally want this in my laboratory. - Doody's Health Sciences Book Review Journal Review of the first edition: The Protein Protocols Handbook has arrived at the right place at the right time. - Trends in Cell Biology From Reviews of the First Edition " a must for researchers in protein chemistry and especially those with less experience " - Doody's Health Sciences Book Review Journal " a very useful tool for daily work.." - Nahrung-Food " definitely an indispensable benchtop handbook highly recommended for all levels of research within the field." - Bioseparation " stands out in an already overcrowded field " - Aslib Book Guide This book is a must-have' for any laboratory involved in analysing proteins...it covers an astonishing range for a book. The chapters have been written by scientists with years of practical experience at the bench, whose enthusiasm for their subject oozes out of the pages. - Annals of Clinical Biochemistry