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This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.
1. Introduction
2. Monochromatic wave propagation
3. Monochromatic field propagation through lens
4. Intensity propagation
5. 3D imaging
6. Radiometry
7. Intensity fluctuations
8. Detection noise
9. Absorption and scattering
10. Widefield microscopy
11. Interference microscopy
12. Optical coherence tomography
13. Fluorescence
14. Confocal microscopy
15. Structured illumination microscopy
16. Multiphoton microscopy
17. Multiharmonic microscopy
18. Pump-probe microscopy
19. Superresolution
20. Imaging in scattering media
Appendix A. Properties of Fourier transforms
Appendix B. Miscellaneous math
Appendix C. Jones matrix description of polarization
Jerome Mertz is a Professor of Biomedical Engineering and Director of the Biomicroscopy Laboratory at Boston University. Prior to joining Boston University, he was a CNRS researcher at the Ecole Superieure de Physique et de Chimie Industrielles in Paris. He is a Fellow of the Optical Society (OSA) and the American Institute for Medical and Biological Engineering (AIMBE).
"Of the many books available on microscopy, my favorite has been Introduction to Optical Microscopy by Jerome Mertz. Now this excellent book is available in an even better second edition."
– Joseph W. Goodman, Stanford University, California
"For advanced undergraduates, graduate students, researchers and engineers who want to gain indepth understanding of the principles of optical microscopy or develop their own microscopes, this book is a must-have."
– Yang Liu, University of Pittsburgh
"No other book covers microscopy theory as broadly as Mertz's, nor with his consistent focus on its theoretical underpinnings. His book fills a clear pedagogical gap in the literature, and is an excellent resource for professionals as well as students new to the field."
– Neil Switz, San Jose State University
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