800 pages, 374 colour illustrations
Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology provides an essential guide to the key techniques used in current bioscience research.
From reviews of the previous editions:
"This book deserves a place in any modern pathology department and brings together a body of information which is traditionally scattered across several texts [...] it also represents excellent value for money."
– Annals of Clinical Biochemistry
"It is necessary to have a practical up to date guide to experimental techniques at hand in a laboratory. This book belongs to such kind of texts. It covers all branches of basic as well as advanced techniques. The book represents a fundamental guidebook for work in a laboratory."
"The book is well illustrated, indexed, free of errors and most chapters have problem sets with worked answers. The book is written for 'all bioscience undergraduate students and pre-medical students for whom practical biochemistry, molecular biology and immunology form part of the syllabus' [...] it is a very good supplement for undergraduate courses in general biochemistry or a text for courses in biochemical techniques. [This book] is also recommended for graduate students and other researchers in applied sciences such as food biochemistry."
– Journal of Food Biochemistry
"[...] well-written and comprehensive [...] what truly distinguishes this text from others of its kind are the outstanding chapters on proteins, mass spectrometry and spectroscopic techniques. [...] a valuable resource for undergraduate or graduate students taking introductory or advanced techniques/laboratory courses in cell/molecular biology, biochemistry or biotechnology."
– Microbiology Today
"I found this volume remarkably satisfying in that it answered all those 'why do you do it like this?' type questions. [...] This book has already received extensive reference value on my bookshelf."
– Journal of Biological Education
Foreword Keith Wilson and John Walker
Preface Samuel Clokie and Andreas Hofmann
Tables and resources Andreas Hofmann
1. Life science studies Samuel Clokie and Andreas Hofmann
2. Basic principles Parisa Amani and Andreas Hofmann
3. Cell culture techniques Anwar Baydoun
4. Recombinant DNA techniques and molecular cloning Ralph Rapley
5. Preparative biochemistry Samuel Clokie
6. Electrophoresis Ralph Rapley
7. Immunochemical techniques Katja Fischer
8. Flow cytometry John Grainger and Joanne Konkel
9. Radioisotope techniques Robert Slater
10. Clinical biochemistry Gillian Rumsby
11. Microscopy Stephen Paddock
12. Centrifugation Kay Ohlendieck and Stephen Harding
13. Spectroscopic techniques Anne Simon and Andreas Hofmann
14. Basic techniques probing molecular structure and interactions Anne Simon and Joanne Macdonald
15. Mass spectrometric techniques Sonja Hess and James MacRae
16. Fundamentals of bioinformatics Cinzia Cantacessi and Anna Protasio
17. Fundamentals of cheminformatics Paul Taylor
18. The Python programming language Timothy Stevens
19. Processing and visualisation of experimental data Jean-Baptiste Cazier
20. Fundamental genome sequencing and annotation Pasi Korhonen and Robin Gasser
21. Fundamentals of proteomics Sonja Hess and Michael Weiss
22. Fundamentals of metabolomics James MacRae
23. Enzymes and receptors Megan Cross and Andreas Hofmann
24. Drug discovery and development David Camp
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Andreas Hofmann is Associate Professor at the Structural Chemistry Program Leader at Griffith University's Eskitis Institute and an Honorary Senior Research Fellow at the Faculty of Veterinary and Agricultural Sciences at the University of Melbourne. He is also fellow of the Higher Education Academy (UK). Hofmann's research lab focusses on the structure and function of proteins in infectious and neurodegenerative diseases, and also develops computational tools. He is the author of Methods of Molecular Analysis in the Life Sciences (Cambridge, 2014) and Essential Physical Chemistry (2017).
Samuel Clokie is the principal bioinformatician at the Birmingham Women's Hospital, where he leads the implementation of bioinformatic techniques to decipher next generation sequencing data in clinical diagnostics. He is also an Honorary Senior Research Fellow at the Institute of Cancer and Genomic Sciences at the University of Birmingham and before that was a Research Fellow at the National Institutes of Health in Bethesda.
- Keith Wilson
- John Walker
- Samuel Clokie
- Andreas Hofmann
- Parisa Amani
- Anwar Baydoun
- Ralph Rapley
- Katja Fischer
- John Grainger
- Joanne Konkel
- Robert Slater
- Gillian Rumsby
- Stephen Paddock
- Kay Ohlendieck
- Stephen Harding
- Anne Simon
- Joanne Macdonald
- Sonja Hess
- James MacRae
- Cinzia Cantacessi
- Anna Protasio
- Paul Taylor
- Timothy Stevens
- Jean-Baptiste Cazier
- Pasi Korhonen
- Robin Gasser
- Michael Weiss
- Megan Cross
- David Camp