Ferns existing today represent a genetic inheritance of great value as they include species of ancient vascular plants, which have direct connection with the crucial steps done in the past for settling life on Earth. Their life cycle is an example of alternation of generations, in which both a multicellular diploid organism and a multicellular haploid organism occur and give rise to the other. Spore, gametophyte and sporophyte have been studied either for basic and practical purposes. In the first case, they offer us simple and easy to grow experimental systems to investigate on plant development, and secondly, they are culture by their ornamental appeal, their environmental benefit or as source of metabolites. This timely volume brings a selection of chapters, each one composed by experts in their respective fields. The chapters included cover a broad range from the knowledge of its biology and contribution to understanding of plant development, useful protocols for propagation and conservation purposes, genetic variability as well as environmental and theurapeutical applications. This wide spectrum of the contributions gives to reader a rapid idea of the enormous potential of this plant group. The originality of Working with Ferns is to expose the most recent tendencies in their investigation, which is far from the traditional perspective usually followed. The collected articles in Working with Ferns incorporate most of novel techniques used nowadays routinely to resolve traditional questions.
Chapter 1: Introduction
CONTRIBUTION OF FERNS TO UNDERSTANDING OF PLANT DEVELOPMENT
Chapter 2 Cellular, molecular and genetic changes during the development of Ceratopteris richardii gametophytes Authors: Salmi M, Bushart T and Roux S J
Chapter 3 Laboratory-induced Apogamy and Apospory in Ceratopteris richardii Authors: Cordle AR., Bui LT, Irish EE, and Cheng C-L
Chapter 4 Sexual reproduction in ferns Authors: Menendez V, Revilla A, Peredo E, Revilla MA, and Fernandez H
Chapter 5 Gibberellic acid and ethylene control male sex determination and development of Anemia phyllitidis gametophytes Author: Kazmierczak A
Chapter 6 The sporophytes of seed-free vascular plants -major vegetative developmental features and molecular genetic pathways Authors: Tomescu A
PROPAGATION, CONSERVATION AND CONTROL OF GENETIC VARIABILITY IN FERNS
Chapter 7 Form spore to sporophyte: How to proceed in vitro Authors: Menendez V, Arbesu R, Somer M, Revilla A, and Fernandez H
Chapter 8 In vitro regeneration systems of Platycerium Authors: Camloh M and Ambrozic-Dolinsek J
Chapter 9 Stipule propagation in five Marattioid species native to Taiwan (Marattiaceae; Pteridophyta) Authors: Huang Y-M, Huang M-H, Chen C-M, Chiou W-L
Chapter 10 Tree ferns biotechnology: from spores to sporophytes Authors: RybczyA ski JJ and MikuACa A
Chapter 11 In Vitro Propagation of Rare and Endangered Serpentine Fern Species Authors: MarszaAC-Jagacka J and Kromer K
Chapter 12 Conservation of fern spores. Authors: Ballesteros D
Chapter 13 Exploration of cryo-methods to preserve tree and herbaceous fern gametophytes Authors: MikuACa A, Makowski D, Walters C, and RybczyA ski JJ
Chapter 14 Pteridophyte spores viability Authors: Gabriel y Galan JM, and Prada C
Chapter 15 Microsatellites: a powerful genetic marker for fern research Author: Jimenez-Soria A
Chapter 16 Diversity in natural fern populations: dominant markers as genetic tools. Authors: Peredo EL, Revilla MA, Mendez M, Menendez V and Fernandez H
ENVIRONMENTAL BIOTECHNOLOGY: ECOTOXICOLOGY AND BIOREMEDIATION IN FERNS
Chapter 17 Mitochondrial activity of fern spores for the evaluation of acute totoxicity in higher plant development Authors: Catala M, Esteban M and Garcia L
Chapter 18 Chronic phytotoxicity in gametophytes: DNA as biomarker of growth and chlorophyll autofluorescence as biomarker of cell function Authors: Catala M and Rodriguez-Gil JL
Chapter 19 Arsenic hyperaccumulator fern Pteris vittata: Utilities for arsenic phytoremediation and plant biotechnology Author: Rathinasabapathi B
Chapter 20 Aerobiology of Pteridophyta spores: preliminary results and applications Author: Rodriguez de la Cruz D
Chapter 21 Studies on Folk Medicinal Fern: An example of "Gusuibu" Author: Chang H-C, Gupta SK and Tsay H-S
Chapter 22 Ecdysteroids in Ferns: diversity, distribution, biosynthesis and functions Authors: Lafont R, Ho R, Raharivelomanana P and Dinan L
Chapter 23 Ferns - from traditional uses to pharmaceutical development; chemical identification of active principles Authors: Ho R, Teai T, Bianchini JP, Lafont R and Raharivelomanana P
Chapter 24 Functional activities of ferns for human health Authors: Lee CH and Shin SL
Chapter 25 Toxicological and medicinal aspects of the most frequent fern species Pteridium aquilinum Author: Vetter J
Helena Fernández Associate Professor, Department of Biology of Organisms and Systems, Oviedo University, Spain. Her research focuses on micropropagation and reproduction in ferns during the two last decades. In 2002 she obtained the award "Ramon y Cajal" by The Ministry of Science and Technology, being engaged to the Oviedo University as tenurer, full time, in the Area of Plant Physiology since then.
Ashwani Kumar Professor, Department of Botany, University of Rajasthan, Jaipur. The author's repertoire of published works spreads across 150 research articles in various national and international journals. He has an experience of over three decades in his field of research, namely, tissue culture and biochemistry, being awarded with prestigeous V. Puri Medal in 2008 for his services to advancement of Botany.
M. Angeles Revilla Plant Physioloy Assistant Professor in the Biology Faculty at the Oviedo University (Spain) since 1987. Twenty years experience in plant tissue culture. She has also worked in cryopreservation and genetic stability for the last ten years, mainly in the development of protocols for in vitro shoot apices in agronomic species.