Protocols for Neural Cell Culture

About this book

This second edition of a best-selling and highly praised collection of unfailingly reproducible methods includes key techniques to study the development and differentiation of the nervous system, new methods for isolating and propagating progenitor cells that can be stimulated to differentiate into either neurons or various types of glial cells, and timely procedures for eliminating contaminating neural or nonneural cells. Important new procedures for the transfection of cells in culture with nontoxic mutant herpes simplex virus are also included, as are essential procedures for quantification, growth assays, evaluating neuroactive agents, preparing neural material for electron microscope analysis, and dissection. This book's unique wealth of practical detail and its inclusion of a wide range of tissue culture systems having many applications ensure that it will remain a valuable resource that will find its place on many laboratory shelves (Trends in Biotechnology) .

Contents

Introduction, Marston Manthorpe. Outgrowth Assays and Cortical Slice Cultures, Valerie Castellani and Jurgen Bolz. Microexplant Cultures of the Cerebellum, Bernard Rogister and Gustave Moonen. Aggregating Neural Cell Cultures, Paul Honegger and Florianne Monnet-Tschudi. Primary Cell Cultures of Peripheral and Central Neurons and Glia, Mary I. Johnson and Richard P. Bunge. Chick Spinal Somatic Motoneurons in Culture, Bernhard H. J. Juurlink. Neural Cells in Microcultures: In Vitro Biological Assays for Neuroactive Agents, Marston Manthorpe. Construction and Use of Compartmented Cultures, Robert B. Campenot. Astrocyte and Oligodendrocyte Cultures, Ruth Cole and Jean de Vellis. Generation of Mouse Astroglia and Microglia Cultures from Mouse Neopallium, Sergey Fedoroff and Arleen Richardson. Generation of Oligodendroblasts from Primary Cultures of Rat Neopallium, Bernhard H. J. Juurlink, Shawn K. Thorburne, and Richard M. Devon. Culture of Glial Cells from Human Brain Biopsies, Voon Wee Yong and Jack P. Antel. Colony Cultures, Sergey Fedoroff and Arleen Richardson. Herpes Simplex Virus Vectors for Gene Therapy of the Nervous System , Angela P. Dyer and Frank Tufaro. Immunostaining and Identification of Antigens, Colin J. Barnstable. Elimination of Cell Types from Mixed Neural Cell Cultures, Richard M. Devon. Quantification of Cells in Culture, Arleen Richardson and Sergey Fedoroff. Procedures for Subculturing Cells, Arleen Richardson and Sergey Fedoroff. Preparation of Tissue-Cultured Material for Electron Microscopical Observation, Richard M. Devon. Tips in Tissue Culture, Sergey Fedoroff and Arleen Richardson. Use of Dissecting Instruments in Tissue Culture, Arleen Richardson. Abbreviations and Definitions. Index.

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