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Microarray Technology provides information in designing, fabricating arrays and binding studies with biological analytes. Divided into two volumes, the first deals with methods for preparations of microarrays, with the second focusing on applications and data analysis. The volumes also provide the reader with a broader description of microarray technology tools and its potential applications. In combination, these volumes will provide valuable information to both novices and experts in the field of research.
Introduction.- Current Microarray Surface Chemistries.- Non-Fouling Surfaces.- Optimization of Oligonucleotide DNA Microarray.- Detection of DNA Copy Number Alterations in Complex Genomes Using Array CGH.- Evaluating the Quality of Data Microarray Measurements.- Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents.- Choice of Polymer Matrix, its Functionalization and Estimation of Functional Group Density for Preparation of Biochips.- Methods in High-Resolution, Array-Based Comparative Genomic Hybridization.- Design and Fabrication of Spotted Long Oligonucleotide Microarrays for Gene Expression.- In-Situ Oligonucleotide Arrays on Plastic.- Detecting Ligated Fragments on Oligonucleotide Microarrays - Optimizing Chip Design, Array MLPA Modification and Hybridization Parameters.- Detection of SNPs in Cancer Related Genes by Minisequencing on a Microelectronic DNA Chip.- Hybridization Analysis Using Oligonucleotide Probe Arrays.- In situ Synthesis of Peptide Microarrays Using Ink-Jet Microdispensing.- Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays.- Printing Low Density Protein Arrays in Microplates.- Protein Microarray Applications - Forward and Reverse Protein Arrays.- Cell Microarray for Functional Exploration of Genomes.- Quantification of Mixed-phase Hybridization on Polymer Microparticles by Europium (III) Ion Fluorescence.- Measurement of the Sugar-Binding Specificity of Lectins Using Multiplexed Bead-Based Suspension Arrays.- Nanotechnology.