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Pyrosequencing Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing.
Table of contents; Chapter 1: The History of Pyrosequencing[registered]. Pal Nyren; Chapter 2: Pyrosequencing[registered] applications. Sharon Marsh; Chapter 3: Web-Based Primer Design Software for Genome Scale Genotyping by Pyrosequencing[registered]. Steven Ringquist, Christopher Pecoraro, Ying Lu, Alexis Styche, William A. Rudert, Panayiotis V. Benos and Massimo Trucco; Chapter 4: Pyrosequencing[registered]: A Simple Method for Accurate Genotyping. Cristi R. King and Tiffany Scott-Horton; Chapter 5: Universal Primer Applications for Pyrosequencing[registered]. Dong-chuan Guo and Dianna M. Milewicz; Chapter 6: Allele Quantification and DNA Pooling Methods. Jonathon Wasson; Chapter 7: Multiplex Pyrosequencing[registered] for DNA Variation Analysis. Pritesh Patel, Yih-Horng Shiao, and Paolo Fortina; Chapter 8: Analysis of gene-specific DNA methylation patterns by the Pyrosequencing[registered] technology. Jorg Tost and Ivo Glynne Gut; Chapter 9: Detecting mutations that confer oxazolidinone resistance in Gram-positive bacteria. Neil Woodford, Sarah E. North and Matthew J. Ellington; Chapter 10: Pyrosequencing[registered] Based Strategies for Improved Allele Typing of HLA Loci. Steven Ringquist, Alexis Styche, William A. Rudert, and Massimo Trucco; Chapter 11: Pyrosequencing[registered] of Phage Display Libraries for the Identification of Cell Specific Targeting Ligands. Ahad A. Rahim; Chapter 12: Gene Copy Number Detection in Animal Studies. Gerli Pielberg and Leif Andersson; Chapter 13: Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing[registered]. Hua Wang and Steven C. Elbein; Chapter 14: Pyrosequencing[registered] Genotype Storage Techniques. Derek J. Van Booven.