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During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants.
As part of the highly successful "Methods in Molecular Biology" series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls. Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study.
1. Multiple Dimensions in Plant Signal Transduction: An Overview Jorge J. Casal 2. Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions Cordelia Bolle 3. Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis Cordelia Bolle 4. Accurate Real-time Reverse Transcription Quantitative PCR Marco Klatte and Petra Bauer 5. Probing Spatio-temporal Intracellular Calcium Variations in Plants Axel Mithofer, Christian Mazars and Massimo E. Maffei 6. Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy Andreas J. Meyer and Thorsten Brach 7. Imaging of Reactive Oxygen Species in vivo Steven M. Driever, Michael J. Fryer, Philip M. Mullineaux and Neil R. Baker 8. Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single Cysteine Mutant-immobilized Resin Ken Motohashi, Patrick G. N. Romano, and Toru Hisabori 9. Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes Julia P. Vainonen, Alexander V. Vener and Eva-Mari Aro 10. Examining Protein Stability and Its Relevance for Plant Growth and Development Claus Schwechheimer, Bjorn C. Willige, Melina Zourelidou and Esther M. N. Dohmann 11. Probing Circadian Rhythms in Chlamydomonas rheinhardtii by Functional Proteomics Volker Wagner and Maria Mittag 12. Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells Katia Schutze, Klaus Harter and Christina Chaban 13. Fluorescence Cross-Correlation Spectroscopy of Plant Proteins Hideki Muto, Masataka Kinjo and Kotaro T. Yamamoto 14. The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin-system (mbSUS) Christopher Grefen, Petr Obrdlik and Klaus Harter 15. Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways Norbert Mehlmer, Elisabeth Scheikl-Pourkhalil and Markus Teige 16. Phosphatase Activities Analyzed by in vivo Expressions Alois Schweighofer, Zahra Ayatollahi and Irute Meskiene 17. Chromatin Immunoprecipitation Experiments to Investigate in vivo Binding of Arabidopsis Transcription Factors to Target Sequences Benjamin Fode and Christiane Gatz 18. Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins Sebastian Steiner and Thomas Pfannschmidt 19. Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay Dierk Wanke and Klaus Harter 20. Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation Kenneth W. Berendzen, Klaus Harter and Dierk Wanke 21. RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System Jan C. Schoning and Dorothee Staiger
From the reviews: "This text pertinently addresses the field of signal transduction in plants. ! This volume covers a wide range of basic and cutting edge techniques that can be used in any number of areas of plant signaling research. ! This volume ! covers a wide range of cutting edge techniques that are leading to novel insight into signal transduction pathways in plant biology. ! this is a fine reference text that surveys a range of current plant experimental tools and techniques." (Beronda L. Montgomery, Plant Science Bulletin, October, 2009)