To see accurate pricing, please choose your delivery country.
United States
All Shops

British Wildlife

8 issues per year 84 pages per issue Subscription only

British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists. Published eight times a year, British Wildlife bridges the gap between popular writing and scientific literature through a combination of long-form articles, regular columns and reports, book reviews and letters.

Subscriptions from £33 per year

Conservation Land Management

4 issues per year 44 pages per issue Subscription only

Conservation Land Management (CLM) is a quarterly magazine that is widely regarded as essential reading for all who are involved in land management for nature conservation, across the British Isles. CLM includes long-form articles, events listings, publication reviews, new product information and updates, reports of conferences and letters.

Subscriptions from £26 per year
Academic & Professional Books  Organismal to Molecular Biology  Genetics & Genomics

CRISPR Gene Editing Methods and Protocols

Handbook / Manual
Series: Methods in Molecular Biology Volume: 1961
By: Yonglun Luo(Editor)
362 pages, 61 colour & 15 b/w photos and illustrations, tables
Publisher: Humana Press
CRISPR Gene Editing
Click to have a closer look
  • CRISPR Gene Editing ISBN: 9781493991693 Hardback Mar 2019 Not in stock: Usually dispatched within 1-2 weeks
Price: £159.99
About this book Contents Customer reviews Related titles

About this book

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.


Part I: Methods for CRISPR-gRNA Design and Quantification of Activity
1. CRISPR-gRNA Design / Maria Pallares Masmitja, Nastassia Johanna Knoedlseder, and Marc Guell
2. Tracking CRISPR's Footprints / Lin Lin and Yonglun Luo
3. Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER / Eva Karina Brinkman and Bas van Steensel
4. Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA / Saskia Koenig, Yang Zhang, Hans Heugh Wandall, Claudio Mussolino, and Eric Paul Bennett
5. Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check / Lin Lin and Yonglun Luo

Part II: Methods for CRISPR Delivery
6. CRISPR-Cas9 Delivery by Artificial Virus (RRPHC) / Suleixin Yang, Qinjie Wu, Yuquan Wei, and Changyang Gong
7. Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery / Laura Barrett Ryo, Emil Aagaard Thomsen, and Jacob Giehm Mikkelsen
8. Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors / Julia Fakhiri, Manuela Nickl, and Dirk Grimm
9. Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs / Anders Laustsen and Rasmus O. Bak

Part III: CRISPR Gene Editing in Human iPSCs
10. Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 / Saniye Yumlu, Sanum Bashir, Jurgen Stumm, and Ralf Kuhn
11. Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes / Michaela Bruntraeger, Meg Byrne, Kathleen Long, and Andrew R. Bassett
12. Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9 / Kirsten E. Snijders, James D. Cooper, Ludovic Vallier, and Alessandro Bertero

Part IV: CRISPR Gene Editing in Other Cell Types
13. CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells / Daria Sergeeva, Jose Manuel Camacho-Zaragoza, Jae Seong Lee, and Helene Faustrup Kildegaard
14. Rapid and Efficient Gene Deletion by CRISPR/Cas9 / Signe Neldeborg, Lin Lin, Magnus Stougaard, and Yonglun Luo
15. Genome Editing in Mice / Lisbeth Ahm Hansen and Ernst-Martin Fuchtbauer
16. CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol / Xi Xiang, Conghui Li, Xi Chen, Hongwei Dou, Yong Li, Xiuqing Zhang, and Yonglun Luo
17. Gene Editing in Primary Cells of Cattle and Pig / Petra Vochozkova, Kilian Simmet, Eva-Maria Jemiller, Annegret Wunsch, and Nikolai Klymiuk

Part V: CRISPR Gene Therapy and Screening
18. Toward In Vivo Gene Therapy Using CRISPR / Kristian Alsbjerg Skipper and Jacob Giehm Mikkelsen
19. CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery / Andreas Holmgaard, Sidsel Alsing, Anne Louise Askou, and Thomas J. Corydon
20. In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection / Francesco Niola, Frederik Dagnaes-Hansen, and Morten Froedin
21. CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes / Emil Aagaard Thomsen and Jacob Giehm Mikkelsen

Customer Reviews

Handbook / Manual
Series: Methods in Molecular Biology Volume: 1961
By: Yonglun Luo(Editor)
362 pages, 61 colour & 15 b/w photos and illustrations, tables
Publisher: Humana Press
Current promotions
New and Forthcoming BooksNHBS Moth TrapBritish Wildlife MagazineBuyers Guides