224 pages, B/w photos and illus
This second edition of a best-selling and highly praised collection of unfailingly reproducible methods includes key techniques to study the development and differentiation of the nervous system, new methods for isolating and propagating progenitor cells that can be stimulated to differentiate into either neurons or various types of glial cells, and timely procedures for eliminating contaminating neural or nonneural cells. Important new procedures for the transfection of cells in culture with nontoxic mutant herpes simplex virus are also included, as are essential procedures for quantification, growth assays, evaluating neuroactive agents, preparing neural material for electron microscope analysis, and dissection. This book's unique wealth of practical detail and its inclusion of a wide range of tissue culture systems having many applications ensure that it will remain a valuable resource that will find its place on many laboratory shelves (Trends in Biotechnology) .
The authors...lay strong laboratory background for a wide variety of studies. This approach makes the book a pleasure to read, besides its immediate value as a reference source for routine work. The book is indispensable for every project in neuroscience. Four Stars! -Doody's Health Sciences Book Review Journal
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