Books  Animal & General Biology  Biochemistry & Molecular Biology 

Bioluminescence Methods and Protocols

Handbook / ManualOut of Print

Series: Methods in Molecular Biology

Edited By: Robert A LaRossa

Humana Press

Hardback | Dec 1998 | #86617 | ISBN: 0896035204
Out of Print Details

About this book

A state-of-the-art collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors - all hands-on researchers who have perfected their protocols to a high degree of reproducibility - illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods are also included for the construction of other recombinant organisms.

... this book is a good review of the use of recombinant DNA technology associated with bioluminescence methods. - Analyst


Part I. The Basics. Improvements in the Application of Firefly Luciferase Assays Sharon R. Ford and Franklin R. Leach. Visualization of Bioluminescence Amy Cheng Vollmer. Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gene Steven R. Kain Guohong Zhang Vanessa Gurtu and Paul A. Kitts. Part II. Analytical Biochemistry. Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue Eberhard Jungling Helmut Kammermeier and Yvan Fischer. Bioluminescent Assay of the Guanylates Sharon R. Ford and Franklin R. Leach. Bioluminescent Assay of the Adenylate Energy Charge Sharon R. Ford and Franklin R. Leach. Part III. Molecular Biology Tools. Photorhabdus luminescent luxCDABE Promoter Probe Vectors Tina K Van Dyk and Reinhardt A. Rosson. Insertion of Promoter Region: luxCDABE Fusions Into the Escherichia Coli Chromosome David A. Elsemore. Probing for Promoters With Luciferase- Transposons David C. Alexander and Michael S. DuBow. Cryopreservation and Reawakening L. Winona Wagner and Tina K. Van Dyk. Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists Vanitha Thulasiraman and Robert Matts. Part IV. Cell-Based Assays. Genotoxic Sensors Amy Cheng Vollmer. Stress Detection Using Bioluminescent Reporters of the Heat -Shock Response Tina K. Van Dyk. Real-Time Reporter of Protein Synthesis Inhibition Matti Korpela Marko Virta and Matti Karp. Luminescence-Based Cell Viability Testing Ian A. Cree. Phagocyte Chemiluminescence Ian A. Cree. Detection of Oxidants Using lux Fusions to Oxidative Stress Promoters Shimshon Belkin. Part V. Environmental Applications. Luciferase-Based Measurement of Water Contaminants Michael A. Costanzo Julie Guzzo and Michael S. DuBow. Bioluminescence-Based Metal Detectors Marko Virta Sisko Tauriainen and Matti Karp. Luminescence Facilitated Detection of Bioavailable Mercury in Natural Waters Tamar Barkay Ralph R. Turner Lasse D. Rasmussen Carol A. Kelly and John W. M. Rudd. A Panel of Stress-Responsive Luminous Bacteria for Monitoring Wastewater Toxicity Shimshon Belkin. Organic Contaminant Detection and Biodegration Characteristics Robert S. Burlage. Detection of Firefly Luciferase-Tagged Bacteria in Environmental Samples Annelie Moller and Janet K. Jansson. Monitoring of GFP-Tagged Bacteria Cells Riccardo Tombolini Janet K. Jansson.

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