A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.
... a valuable update on the technology, with emphasis on methods for micropropagation and gene transfer...the editor has assembled an excellent range of expert authors and the volume is well balanced and up-to-date...thoroughly recommended. - Plant Growth Regulation Protocols are presented for many agronomically important plants including the culture and regeneration of rice, potato, barley, casava, spruce, Pasiflora, Lolium, and various species of Brassica. The organization of all of the chapters is consistent including an introduction, a materials section divided into appropriate subsections, a similarly subdivided methods section, a notes section that contains important observations to make or precautions to take, and a final reference section...the techniques are well written and include specific examples and step-by-step methods with appropriate references...will make an excellent addition to the library of researchers who wish to extend their collection of basic plant cell culture techniques. - Quarterly Review of Biology The combination of many years of experience with new experimental entries is often very successful and of high quality represents one of the highest quality manuals on this topic published with the last ten years it will assure the relevance of an important scientific discipline, plant biotechnology, in today's world. - Biologia Plantarum The book brings together a series of well written, step-by-step protocols on the most frequently used techniques in plant cell and tissue culture, covering various aspects of culture initiation, maintenance, long-term storage and manipulation. In addition to protocols on widely applied methods, each part includes representative protocols for more specialized techniques. All the chapters are well structured, profusely illustrated with original photographs and line drawings, and contain detailed descriptions of materials, culture media and comprehensively described experimental procedures. The reader will certainly appreciate the precautionary notes and additional hints on each technique and each protocol provided at the end of each chapter. - Elsevier This book presents a comprehensive collection of applicable techniques for plant cell and tissue culture. The methods cover culture initiation, maintenance, manipulation, application and long-term storage, with emphasis on techniques for genetic modification and micropropagation. - Lavoisier Librairie
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