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This is the first text of its kind to address the breadth of the field of biopreservation - the functional maintenance of biologically derived products. The book expertly integrates recent molecular-based discoveries with classically-based biopreservation basics, offering a new look at advances in the emerging regenerative medicine arena. In addition to general concepts of biopreservation, it includes chapters detailing subspecialty principles, such as biologically active macromolecules, stem cells, and gene therapy, as well as new protocols for cell, tissue, and organ preservation. This is a fundamental "how-to" guide for anyone involved in the biopreservation sciences.
Concepts in Biopreservation. Fundamentals of Hypothermic Preservation. Properties of Cells & Tissues Influencing Preservation Outcome: Molecular Basis of Preservation Induced Cell Death. Selective Cryotherapy: Preservation-Ablation. Biomaterials.Viability and Functional Assays Used to Assess Preservation. Preservation of Cellular Therapies: Gene Therapy, Cellular and Metabolic Engineering.Tissue Preservation. Organ Preservation for Transplantation. Cryopreservation of Non-Mammalian MetazoanSystems. The Use of Intracellular Protectants in Cell Biopreservation. Thermal Instruments and Devices in Cryobiological Research and Applications. Solid Mechanics Aspects of Cryobiology. Stabilization of Mammalian Cells in the Dry State.