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This book provides an image rich documentation of the Eunotia flora of the Great Smoky Mountains National Park, USA. Over 50 sub-generic taxa of Eunotia are presented, including 14 proposed new species. Image plates include both light and scanning electron micrographs showing size ranges, morphological variability, and external and internal valve ultrastructure. The taxonomic section includes morphological measurements and some autecological information for each taxon. These data provide a useful resource guide that contributes to our understanding of the Eunotia flora of North America, and will facilitate biomonitoring efforts and taxonomic comparisons with past and recent Eunotia flora descriptions from around the world.