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Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of Molecular Biomethods Handbook describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up-to-date collection of methods used regularly in the authors own research programs. Molecular Biomethods Handbook will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.
Molecular Biomethods Handbook 2nd Ed Contents PART A: NUCLEIC ACID BIOMETHODS 1 The manipulation of nucleic acids: basic tools and techniques -- Gayle Corkill and Ralph Rapley 2 Restriction Enzymes -- Gareth J.S. Jenkins 3 Principles and Medical Applications of the Polymerase Chain Reaction -- Birmal D.M. Theophilus 4 Probe Design, Production, and Applications -- Marilena Aquino de Muro 5 Southern Blotting as a Diagnostic Method -- Bronwen Harvey and Pirkko Soundy 6 Introduction to Capillary Electrophoresis of DNA - Beatriz Sanchez-Vega 7 Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid analysis - Kim Hung Leung and Shea Ping Yip 8 Denaturing Gradient Gel Electrophoresis (DGGE) -- Jeroen H. Roelfsema and Dorien J.M. Peters 9 Single Strand Conformation Polymorphism (SSCP) Analysis - Kim Hung Leung and Shea Ping Yip 10 Randomly amplified polymorphic DNA (RAPD); a useful tool for genomic characterization of different organisms - Lucia Maria da Cunha Galvao and Eliane Lages-Silva 11 Quantification of mRNA using Real Time RT-PCR -- the SYBR solution - David Sugden and Patricia de Winter 12 Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing -- Anu Suomalainen and Ann-Christine Syvanen 13 MAPH (Multiplex Amplifiable Probe Hybridization) - Carolina Sismani, Ludmila Kousoulidou and Philippos C. Patsalis 14 Gene Expression Profiling - Arnis Druka, Robbie Waugh and Pete Hedley 15 Comparative Genomic Hybridization in Clinical and Medical Research -- Peng-Hui Wang, Yann-Jang Chen and Chi-Hung Lin 16 Subtractive Hybridization -- Craig Winstanley 17 Fluorescence in situ Hybridization - Jane Bayani and Jeremy A. Squire 18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice - Jonathan D. Smith 19 cDNA Microarrays -- Phillip G. Febbo 20 Mapping Techniques -- Simon G. Gregory 21 Single Nucleotide Polymorphisms Overview -- Nameeta Shah 22 Bioinformatics Tools for Gene and Protein Sequence Analysis-- Bernd H.A. Rehm and Frank Reinecke PART B: PROTEIN AND CELL METHODS 23. Protein Electrophoresis - David Sheehan and Siobhan O'Sullivan 24. Protein blotting - Patricia Gravel 25. Capillary electrophoresis of proteins - Mark Strege 26. Autoradiography and fluorography - Bronwen Harvey 27. Mass spectrometry of proteins & peptides - Kenneth G. Standing 28. Post-translational modifications - Christoph Kannicht and Birte Fuchs 29. Protein microarray technology - Charlotte H. Clarke and Eric T. Fung 30. Protein-protein interactions - Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith Wilkinson and Haian Fu 31. Glycoprotein analysis - Terry Butters and David C.A. Neville 32. Solid Phase Peptide Synthesis - Mare Cudic and Gregg B. Fields 33. Monoclonal antibodies - Zhong J. Zhang and Maher Albitar 34. Antibody Phage display - Rob Aitken 35. Protein engineering - Thomas Willemsen, Urs B. Hagemann, Eva M. Jouaux, Sabine Stebel, Jody M. Mason, Kristian M. Muller and Katja M. Arndt 36. Directed protein evolution - Sabine C. Stebel, Annette Gaida, Katja M. Arndt and Kristian M. Muller 37. Enzyme Linked Immunosorbent Assay - John Crowther 38. Epitope mapping - Glenn E. Morris 39. Quantum dots - Charles Hotz 40. Ion exchange chromatography - David Sheehan 41. Size exclusion chromatography - Paul Cutler 42. Hydrophobic interaction chromatography - Paul A. O'Farrell 43. Affinity chromatography - Adam Charlton and Michael Zachariou 44. High Performance Liquid Chromatography (HPLC) of Proteins and Peptides - Tzong-Hsien Lee and Marie-Isabel Aguilar 45. Amino acid analysis - Ian Davidson + Paula O'Connor 46. Surface plasmon resonance - K. Scott Phillips and Quan Cheng 47. Macromolecular Crystallography - Bernhard Rupp and Kathrine A. Kantardjieff 48. Microchip Devices for Bioanalysis - Anna Kinsella and Shelley D. Minteer 49. Mammalian cell culture - Simon Langdon 50. Plant tissue culture - V.M. Loyola Vargas, C. De-la-Pena, R.M. Galaz-Avalos and F. R. Quiroz-Figueroa 51. Stem cells and Regenerative Medicine - Mohan C. Vemuri and Chellu S. Chetty 52. Cryopreservation : Conservation of Bioresources at Ultra-Low Temperatures - J.G. Day, K. Harding, J. Nadarajan and E.E. Benson 53. Magnetic resonance imaging (MRI) - Pottumarthi V. Prasad and Pippa Storey 54. Electron microscopy - John Kuo 55. Confocal microscopy - Guy Cox 56. Laser microdissection - Graeme I. Murray 57. Biomedical uses of Flow cytometry - James L. Weaver and Maryalice Stetler-Stevenson 58. Immunomicroscopy - Constance Oliver 59. In-situ hybridization - Ian A. Darby and Tim D. Hewitson 60. High throughput screening - William P. Janzen
"If you want to know the theoretical basis of how a technique works, how it might be applied, or what techniques are available to allow you to ask a particular question, then this book could prove very useful. [...] this book will be of interest to young (and not so young) scientists who want to better understand the techniques they use, or may wish to use, and would be a useful addition to a library or PhD room."
– Bob Dalziel, Microbiology Today, February, 2009
"The book is a second edition and this includes several new sections which deal with new areas e.g. microarray technology and nanotechnology [...] . These deal with the physical concepts behind the techniques in an accessible way and will be useful to a large number of researchers. [...] In summary, I found the book to be generally comprehensive, clearly written and logically organised. [...] it would be a useful addition to any molecular biology laboratory bookshelf."
– Nicola J Stonehouse, British Toxicology Society Newsletter, June-August, 2009
"Written by experts in their fields, the book gives a wide scope of molecular biology experimentation really living out only the most specialized uncommon techniques while at the same time giving reference to each chapter and sharing full information with readers. This handbook is suitable for research scientists, technicians, and graduate research students in molecular and cell biology, proteomics, and related branches of science."
– G. Ya. Wiederschain, Biochemistry, Vol. 74 (9), 2009