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About this book
About this book
The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant's life. In Plant Developmental Biology: Methods and Protocols, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology, including diverse methods that range from grafting over bimolecular fluorescence complementation to chromatin immunoprecipitation.
Chapters are divided into six major parts: growth protocols, manipulation of gene activity, assaying developmental phenotypes, assaying gene activity, testing protein-protein interactions, and probing chromatin. Composed in the highly successful Methods in Molecular BiologyT series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Plant Developmental Biology Edited by Lars Hennig and Claudia Kohler Contents 1. Growth protocols for model plants in developmental biology Lars Hennig 2. Grafting as a research tool Colin G. N. Turnbull 3. Viral-induced gene silencing as a reverse genetics tool to study gene function Steven Bernacki, Mansour Karimi, Pierre Hilson and Niki Robertson 4. The CRE/lox system as a tool for developmental studies at the cell and tissue level Guy Wachsman and Renze Heidstra 5. Inducible gene expression systems for plants Lorenzo Borghi 6. Trichome development in Arabidopsis Joachim F. Uhrig and Martin Hulskamp 7. Phenotyping the development of leaf area in Arabidopsis thaliana Sarah J. Cookson, Olivier Turc, Catherine Massonnet and Christine Granier 8. Analyzing shoot apical meristem development Cristel Carles, Chan Man Ha, Ji Hyung Jun, Elisa Fiume and Jennifer C. Fletcher 9. Analyzing floral meristem development Elisa Fiume, Helena R. Pires, Jin Sun Kim and Jennifer C. Fletcher 10. Female gametophytic mutants: Diagnosis and characterization Ronny Volz and Rita Gross-Hardt 11. Pollen tube development Mark A. Johnson and Benedikt Kost 12. Analysis of root meristem size development Serena Perilli and Sabrina Sabatini 13. Phenotypic characterization of photomorphogenic responses during plant development Thomas Kretsch 14. Kinematic analysis of cell division and expansion Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabio Fiorani and Gerrit T.S. Beemster 15. Flowering time control Yvonne Moller-Steinbach, Cristina Alexandre and Lars Hennig 16. mRNA detection by whole mount in situ hybridization WISH or sectioned tissue in situ hybridzation SISH in Arabidopsis Yvonne Stahl and Rudiger Simon 17. Immunolocalization of proteins in plants Michael Sauer and JiA'i Friml 18. Detection of small non-coding RNAs Tamas Dalmay 19. Quantitative real time PCR in plant developmental biology Vivien Exner 20. Luciferase and green fluorescent protein reporter genes as tools to determine protein abundance and intracellular dynamics Andras Viczian and Stefan Kircher 21. Fluorescence-activated cell sorting in plant developmental biology Anjali S. Iyer-Pascuzzi and Philip N. Benfey 22. Laser microdissection of paraffin embedded plant tissues for transcript profiling Robert C. Day 23. Utilizing bimolecular fluorescence complementation BiFC to assay protein-protein interaction in plants Nir Ohad and Shaul Yalovsky 24. The split luciferase complementation assay Naohiro Kato and Jason Jones 25. Co-immunoprecipitation and protein blots Erika Isono and Claus Schwechheimer 26. Probing protein-protein interactions with FRET-FLIM Christoph Bucherl, Jose Aker, Sacco de Vries, Jan Willem Borst 27. Plant chromatin immunoprecipitation Corina B. R. Villar and Claudia Kohler 28. Immunocytological analysis of chromatin in isolated nuclei Penka Pavlova, Federico Tessadori, Hans J. de Jong and Paul Fransz 29. Bisulphite sequencing of plant genomic DNA Ernst Aichinger and Claudia Kohler