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The 157 species of dynastine scarab beetles that occur in Costa Rica and Panama are comprehensively reviewed. Bilingual keys, descriptions, distributions, and notes on biology are provided for all species as well as illustrations and maps. The larvae of Enema endymion, Heterogomphus chevrolati, and Dynastes hercules are described for the first time. Also included are synopses of the higher-level taxa, glossary, gazetteer of place names, and species checklist.