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This work is a comprehensive and accessible survey of the best current accomplishments of GMO research in all their complexity and ramifications. The authors introduce the fundamentals of biotechnology as a scientific discipline, show how GMO research is conducted today, discuss the problems that have arisen from genetic technology and the tools needed to resolve them, and describes how GMO-derived technology may impact our lives in the future. On the technical side, the authors examine a wide range of current technologies employed for constructing GMOs, and describe approaches to novel research, appropriate protocols, and the process of constructing and screening a GMO. The discussion of plant and animal cells covers new strategies employed and the large-scale expression and purification of recombinant products in cultured cells. Social political, and legal issues are also discussed.
Part I. Introduction. Introduction. Part II. Microbial GMOs. Genetically Modified Microorganisms: Development and Applications. Producing Proteins Derived From Genetically Modified Organisms for Toxicology and Environmental Fate Assessment of Biopesticides. Genetically Modified Organisms: Biosafety and Ethical Issues. Part III. Mammalian GMOs. Large-Scale Exogenous Protein Production in Higher Animal Cells. Biosafety, Ethics, and Regulation of Transgenic Animals. Transgenic Aquatic Animals. Part IV. Plant GMOs. Development of Genetically Modified Agronomic Crops. Gene Silencing in Plants: Nature's Defense. Value Creation and Capture With Transgenic Plants. Biosafety Issues, Assessment, and Regulation of Genetically Modified Food Plants. Conclusion and Future Directions. Glossary. Index.
...succeeds in providing scientists and advancecd science and engineering students with a starting point for understanding biotechnology...should be on the shelves of most public and private research libraries around the world. -American Entomologist