For both volumes: Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing. An accompanying volume, Volume 2: "Functional Studies", provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
Use of BAC End Sequences for SNP Discovery Michael M. Weil, Rashmi Pershad, Ruoping Wang, and Sheng Zhao Exon Trapping for Positional Cloning and Fingerprinting Scott E. Wenderfer and John J. Monaco Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column Sally H. Cross BAC Microarray-Based Comparative Genomic Hybridization Antoine M. Snijders, Richard Segraves, Stephanie Blackwood, Daniel Pinkel, and Donna G. Albertson Large DNA Transformation in Plants Sangdun Choi Retrofitting BACs With a Selectable Marker for Transfection Zunde Wang, Angelika Longacre, and Peter Engler BAC Modification Using a RecA Expressing Shuttle Vector System Nathan Mise and Philip Avner Bacterial Artificial Chromosome Engineering Srividya Swaminathan and Shyam K. Sharan ET Recombination: DNA Engineering Using Homologous Recombination in E. coli Joep P. P. Muyrers, Youming Zhang, Vladmir Benes, Giuseppe Testa, Jeanette M. J. Rientjes, and A. Francis Stewart BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes Giuseppe Testa, Kristina Vintersten, Youming Zhang, Vladmir Benes, Joep P. P. Muyrers, and A. Francis Stewart Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes Kristina Vintersten, Giuseppe Testa, and A. Francis Stewart Generation of BAC Transgenic Mice Vikki M. Marshall, Janette Allison, Tanya Templeton, and Simon J. Foote BAC Rescue: A Tool for Functional Analysis of the Mouse Genome Deborah A. Swing and Shyam K. Sharan Herpesviruses: A Brief Overview Mathias Ackermann Cloning of b-Herpesvirus Genomes as Bacterial Artificial Chromosomes Eva-Maria Borst, Irena Crnkovic-Mertens, and Martin Messerle Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors Thomas G. H. Heister, Andrea Vogtlin, Lars Muller, Irma Heid, and Cornel Fraefel Mutagenesis of Viral BACs With Linear PCR Fragments (ET Recombination) Markus Wagner and Ulrich H. Koszinowski Mutagenesis of Herpesvirus BACs by Allele Replacement Eva-Maria Borst, Gyorgy Posfai, Frank Pogoda, and Martin Messerle Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies Alistair McGregor and Mark R. Schleiss Protective DNA Vaccination by Particle Bombardment Using BAC DNA Containing a Replication-Competent, Packaging-Defective Genome of Herpes Simplex Virus Type I Mark Suter and Hans Peter Hefti Index
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