In Vitro Toxicity Testing Protocols

About this book

In Vitro Toxicity Testing Protocols is a collection of proven, state-of-the-art methods that toxicologists can use in carrying out laboratory studies. The book's expert contributors offer a variety of efficient in vitro toxicology techniques that can be applied regularly in your lab, or can serve as the fundamental basis for the establishment of novel techniques in working laboratories. All the procedures are highly reliable. Also included are ex vivo techniques, whose development embraces the twin aims of reduction and refinement of the use of laboratory animals; the editors believe this is an important step toward the ethical treatment of laboratory animals. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, concise, easy-to-follow, step-by-step protocols are provided for each technique, together with extensive notes that cover troubleshooting and offer special tips to ensure the successful completion of your experiments.

Contents

Alternative Method of Assessing Toxicity. Target Organ Toxicity: LLC-RK1 Cell Screening Test for Nephrotoxicity. Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity. Human Thyroid Culture. Thyroid Follicular Cells in Monolayer Culture: In Vitro Models for Thyroid Toxicity Testing. Dust Toxicity in Rat Alveolar Macrophage Cultures. Hepatoma Cell Cultures as In Vitro Models for Hepatotoxicity. Cytotoxicity DNA Fragmentation and DNA Repair Synthesis in Primary Human Hepatocytes. Model Cavity Method. Human Esophageal Culture. The Application of In Vitro Models of Anterior Pituitary Function in Toxicity Testing. The Application of In Vitro Models of Hypothalamic Function in Toxicity Testing. General and Topical Toxicity: The FRAME Cytotoxicity Test (Kenacid Blue). Allium Test. Measurement of Cell Membrane Toxicity by Means of 2-Deoxy-D-Glucose. MTT Assays. V79 Cytotoxicity Test for Membrane Damage. SIRC Cytotoxicity Test for Membrane Damage. Rabbit Articular Chondrocyte Functional Toxicity Test. Balb/c 3T3 Cytotoxicity Test. The Pollen Tube Growth Test. Irritancy Testing: HET-CAM Test. The Use of the Bovine Isolated Cornea as a Possible In Vitro Test for Occular Irritancy. Cytotoxicity in an Anchorage-Independent Fibroblast Cell Line Measured by a Combination of Fluorescent Dyes. Cell Culture Phototoxicity Test. Immunotoxicity: The Murine Local Lymph Node Assay. Polymorphonuclear Leukocytes Locomotion. Immune Function Assays. Carcinogenesis: The DNA Alkaline Unwinding Genotoxicity Test. Measurement of Unscheduled DNA Synthesis In Vitro Using Primary Rat Hepatocyte Cultures. Gene Mutation Assays in Mammalian Cells. Measurement of Chromosome Aberrations In Vitro Using Human Peripheral Blood Lymphocytes. Mutation Assays in Bacteria. Submammalian and Subvertebrate Models for Teratogenicity Testing: Chick Embryrotoxicity Screening Test (CHEST I and II). Frog Embryo Teratogenesis Assay: Xenopus (FETAX). The Drosophila melanogaster Assay. The Hydra attenuata Assay. Index.

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