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About this book
The past decade has seen the emergence of a new field of scientific inquiry at the intersection of phylogenetics and genomics: phylogenomics.
This volume is a collection of protocols and resources compiled by leading researchers in the field and describes many of the molecular methods and bioinformatics tools that have brought this field to fruition in recent years.
Contents
From gene-scale to genome-scale phylogenetics; the data flood in but the challenges remain.- Phylogenomic analysis by chromosome sorting and painting.- FISH for mapping single copy genes.- Construction of radiation hybrid panels.- Survey sequencing and radiation hybrid mapping to construct comparative maps.- Construction of high-resolution comparative maps in mammals using BAC-end sequences.- Amniote phylogenomics: testing evolutionary hypotheses with BAC library scanning and targeted clone analysis of large scale sequences from reptiles.- Comparative physical mapping: universal overgo hybridization probe design and BAC library hybridization.- Phylogenomic resources at the UCSC Genome browser.- Computational tools for the analysis of rearrangments in mammalian genomes.- Computational reconstruction of ancestral DNA sequences.- Sequencing and phylogenomic analysis of whole mitochondrial genomes of animals.- Retroposons: Genetic Footprints on the Evolutionary Paths of Life.- LINE-1 elements: analysis by fluorescence in situ hybridization (FISH) and nucleotide sequences.- Identification of cryptic sex chromosomes and isolation of X and Y borne genes.
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