Protein Sequencing Protocols

About this book

Bryan John Smith updates his much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. Comprehensive and up-to-date, "Protein Sequencing Protocols, Second Edition", provides for both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination.

Contents

Strategies for Handling Polypeptides on a Micro-Scale Bryan John Smith and Paul Tempst SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing Bryan John Smith Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization Michael J. Dunn Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization Michael J. Dunn Enzymatic Cleavage of Proteins Bryan John Smith Chemical Cleavage of Polypeptides Bryan John Smith Enzymatic Preparation and Isolation of Glycopeptides David J. Harvey Analytical and Micropreparative Capillary Electrophoresis of Peptides Alan J. Smith High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues Christoph W. Turck Hydrolysis of Samples for Amino Acid Analysis Ian Davidson Amino Acid Analysis: Precolumn Derivatization Methods G. Brent Irvine and Ian Davidson Post Column Amino Acid Analysis Alan J. Smith Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples Steven A. Cohen Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection Petr Jandik, Jun Cheng, and Nebojsa Avdalovic d-Amino Acid Analysis Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa Validation of Amino Acid Analysis Methods Andrew J. Reason Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins Fiona M. Greer and Howard R. Morris Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry Darryl J. C. Pappin Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, and Andrej Shevchenko Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis David M. Schieltz and John R. Yates, III Identification of PTH-Amino Acids by HPLC Gregory A. Grant and Mark W. Crankshaw Protein Sequencer Maintenance and Troubleshooting Bryan Dunbar N-Terminal Protein Sequencing for Special Applications Philip J. Jackson Identification of Phosphorylation Sites by Edman Degradation Laurey Steinke and Richard G. Cook Validation of Protein Sequencing in a Regulated Laboratory Philip J. Jackson and Neil Dodsworth Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham Chemical Modifications of Proteins as an Aid to Sequence Analysis Alex F. Carne Deblocking of N-Terminally Modified Proteins Hisashi Hirano and Roza M. Kamp Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid Jacek Mozdzanowski Identification of Sites of Protein Glycosylation David J. Harvey Analysis of Sites of Protein Phosphorylation Alastair Aitken and Michele Learmonth Quantitation and Location of Disulfide Bonds in Proteins Alastair Aitken and Michele Learmonth Getting the Most from Your Protein Sequence Richard R. Copley and Robert B. Russell Appendix 1: Letter Codes, Structures, Masses, and Derivatives of Amino Acids James P. Turner Appendix 2: Protein Consensus Sequence Motifs Alastair Aitken Index

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