Books  Animal & General Biology  Biochemistry & Molecular Biology 

Handbook of ELISPOT: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 302

By: Alexander Kalyuzhny

Humana Press

Hardback | Dec 2005 | #163640 | ISBN: 1588294692
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £76.50 $93/€85 approx

About this book

In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

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