Books  Animal & General Biology  Biochemistry & Molecular Biology 

Electroporation Protocols for Microorganisms

Handbook / Manual

Series: Methods in Molecular Biology Volume: 47

Edited By: JA Nickloff

400 pages, Illus

Humana Press

Spiralbound | Dec 1995 | #47963 | ISBN: 0896033104
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £108.00 $132/€121 approx

About this book

Examination of one of the most versatile and efficient techniques in molecular genetics today.

...the most comprehensive treatment available on the topic of electroporation of microorganisms...will be a useful resource for scientists working on diverse aspects of microbial genetics. - Four Stars, Doody's Health Sciences Book Review Journal


Electroporation Theory: Concepts and Mechanisms. Instrumentation. Direct Plasmid Transfer Between Bacterial Species and Electrocuring. Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation. Production of cDNA Libraries by Electroporation. Electroporation of RNA into Saccharomyces cerevisiae. Electrofusion of Yeast Protoplasts. Escherichia coli Electrotransformation. Electrotransformation in Salmonella. Electrotransformation of Pseudomonas. Electroporation of Xanthomonas. Transformation of Species with Suicide and Broad Host-Range Plasmids. Electroporation of Francisella tularensis. A Simple and Rapid Method for Transformation of Vibrio Species by Electroporation. Genetic Transformation of Bacteroides spp. Using Electroporation. Electrotransformation of Agrobacterium. Electroporation of Helicobacter pylori. Electrotransformation of Streptococci. Transformation of Lactococcus by Electroporation. Transformation of Lactobacillus by Electroporation. Electrotransformation of Staphylococci. Electroporation and Efficient Transformation of Enterococcus faecalis Grown in High Concentrations of Glycine. Introduction of Recombinant DNA into Clostridium spp. Electroporation of Mycobacteria. Electrotransformation of the Spirochete Borrelia burgdorferi. Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation. Ten-Minute Electrotransformation of Saccharomyces cerevisiae. Electroporation of Schizosaccharomyces pombe. Gene Transfer by Electroporation of Filamentous Fungi. Transformation of Candida maltosa by Electroporation. Electroporation of Physarum polycephalum. Electroporation of Dictyostelium discoideum. Gene Transfer by Electroporation of Tetrahymena. Transfection of the African and American Trypanosomes. Electroporation in Giardia lamblia. Index.

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