Protein NMR Protocols

About this book

Leading experts demonstrate how to successfully apply the fundamentals of NMR to the study of the properties of proteins. They cover not only assignment and structure determination techniques, but also the practical aspects of experimental set-up, protein production, data processing, and binding studies. Extensive treatments of paramagnetics and metalloproteins, membrane-bound proteins, and protein ligand complexes are also included. Its focused treatment of proteins make it an indispensable study guide for chemists and biochemists, including both those with only a basic knowledge of NMR, as well as more experienced investigators.

Contents

Introduction to the NMR of Proteins David G. Reid Lesley K. MacLachlan Andrew J. Edwards Julia A. Hubbard and Patricia J. Sweeney. Double and Triple Resonance NMR Methods for Protein Assignment Brian Whitehead C. Jeremy Craven and Jonathan P. Waltho. Protein Chemical Shifts Michael P. Williamson and Tetsuo Asakura. Data Processing in Multidimensional NMR Istv n Pelczer and Brian G. Carter. Calculating Protein Structures from NMR Data Peter G?ntert. Studies of Protein-Ligand Interactions by NMR David J. Craik and Jacqueline A. Wilce. NMR of Paramagnetic Proteins Michael J. Osborne David Crowe Sharon L. Davy Colin Macdonald and Geoffrey R. Moore. NMR Studies of Peptides and Proteins Associated with Membranes R. Bryan Klassen and Stanley J. Opella. Metal NMR for the Study of Metalloproteins Torbjrn Drakenberg Charlotta Johansson and Sture Fors'n. Production and Characterization of Recombinant Proteins for NMR Structural Studies Danuta E. Mossakowska and Richard A. G. Smith. A Case History: NMR Studies of the Structure of a Small Protein w-Conotoxin MVIIA Lesley K. MacLachlan David A. Middleton Andrew J. Edwards and David G. Reid. Spectrometer Calibration and Experimental Setup: Basic Principles and Procedures Ian M. Brereton. Index.

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