Books  Animal & General Biology  Biochemistry & Molecular Biology 

Protein Targeting Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 390

Edited By: Mark van der Giezen

Humana Press

Hardback | Apr 2007 | Edition: 2 | #163687 | ISBN-13: 9781588297020
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £115.50 $145/€136 approx

About this book

This volume is an up date of the previous edition, Protein Targeting Protocols, placing the emphasis on protein targeting to cellular compartments in both prokaryotic and eukaryotic systems. State-of-the-art information is provided on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol described to follow the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching.

a valuable resource to the molecular biology community. The detailed protocols will greatly aid researchers - The Quarterly Review of Biology


Contents

Surface Plasmon Resonance: Measuring Protein Interactions in Real Time, G. Panayotou. Microcalorimetry of Protein Protein Interactions, A. Cooper. Partitioning of Proteins in Triton X-114, J. G. Pryde. Co-immunoprecipitation: Identification of Interacting Proteins, N. G. Anderson. Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring, Z. E. Hausken, V. M. Coghlan, and J. D. Scott. Size-Exclusion Chromatography: Identification of Interacting Proteins, J. Beattie. Overlay and Bead Assay: Determination of Calcium Channel Subunit Interaction Domains, V. E. S. Scott, C. A. Gurnett, and K. P. Campbell. Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins, M. Harris. Molecular Genetic Approaches I: Two Hybrid Systems, G. B. Bolger. Molecular Genetic Approaches II: Expression Interaction Cloning, M. Csukai and D. Mochly-Rosen. Molecular Genetic Approaches III: Determination of Protein Sequence Motifs Involved in Protein Targeting by Use of Coupled Transcription Translation Systems, G. Scotland and M. D. Houslay. Molecular Genetic Approaches IV: Recombinant Expression of Wild-Type and Acylation-Resistant G-Protein a-Subunits Using Transient Transfection Systems, M. A. Grassie and G. Milligan. Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions, J. D. Scott and M. C. Faux. SH2 and SH3 Domains: Unraveling Signaling Networks with Peptide Antagonists, R. Stein. PH Domain of Serine Threonine Protein Kinase B (RAC-PKB): Expression and Binding Assay for Phosphoinositides and Inositol Phosphates, M. Frech and B. A. Hemmings. Membrane Targeting via Protein N-Myristoylation, R. A. J. McIlhinney. Membrane Targeting via Protein Palmitoylation, M. Veit and M. F. G. Schmidt. Agonist-Mediated Turnover of G-Protein a-Subunit Palmitoyl Groups: Role in Membrane Insertion, M. A. Grassie and G. Milligan. Membrane Targeting via Protein Prenylation, J. H. Overmeyer, R. A. Erdman, and W. A. Maltese. Nuclear Protein Import in a Permeabilized Cell Assay, F. Melchior. Clathrin-Coated Vesicles and Targeting: Preparation of Adaptor Proteins, A. Pauloin. Analysis of the Sorting of Secretory Proteins to the Regulated Secretory Pathway: A Subcellular Fractionation Approach, E. Chanat, A. S. Dittie, and S. A. Tooze. Index.

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