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Confocal Microscopy Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 122

Edited By: Stephen W Paddock

Humana Press

Hardback | Dec 1999 | #98997 | ISBN: 0896035263
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £112.50 $143/€134 approx

About this book

Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging - from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. The powerful hands-on methods collected here will help even the novice to produce first-class cover-quality confocal images.

This book is an essential reference for anyone who is involved in the production of laser scanning confocal (LSCM) images... Exceptionally useful information on a great variety of methodologies that can be employed with LSCM is provided... In consideration of the vast quantity of useful information on all aspects of LSCM that is presented in this volume, and the fact that it is the first comprehensive book on this topic, it is highly recommended. - Quarterly Review of Biology almost every research institute has a confocal microscope, and many are developing much larger multi-user biomedical imaging facilities. The publication of this collection of methods and protocols for confocal microscopy is therefore very timely The tips and tricks used on the various systems should prove useful to a diverse range of confocal microscope users and would be a beneficial reference volume for any imaging unit and reasonable value for money. - Cell Biology International This is an excellent source for anyone who wants to explore techniques in confocal microscopy. It is a practical manual with many gems that will help both new and experienced users. The principles are efficiently and accessibly explained and there is a useful chapter on fluorescent probes there is a wealth of ideas here waiting to be applied to prokaryotic biology and many would be equally useful for simple fluorescence rather than confocal studies. I would certainly recommend it to any lab that uses fluorescence microscopy extensively. - Microbiology Today ...The texts are aimed at the biological user...for the user for whom they are intended, these essays contain a wealth of down-to-earth practical information. The book is well-produced and contains colour illustrations... - Ultramicroscopy excellent information as an introductory basis for confocal microscopy. - Cellular and Molecular Biology


Contents

An Introduction to Confocal Imaging Stephen W. Paddock. Practical Considerations for Collecting Confocal Images David Carter. Fluorescent Probes for Confocal Microscopy Christopher Cullander. Imaging Gene Expression Using Antibody Probes Nadean L. Brown. Single and Double FISH Protocols for Drosophila Sarah C. Hughes and Henry M. Krause. Confocal Microscopy of Plant Cells Carol L. Wymer Alison F. Bevan Kurt Boudonck and Clive W. Lloyd. Preparation of Yeast Cells for Confocal Microscopy Audrey Atkin. Confocal Methods for C. elegans Sarah L. Crittenden and Judith Kimble. Imaging Sea Urchin Fertilization Jon M. Holy. Imaging Immunolabelled Drosophila Embryos by Confocal Microscopy James Langeland. Confocal Microscopy on Xenopus laevis Oocytes and Embryos Denise L. Robb and Chris Wylie. Analyzing Morphogenetic Cell Behaviors in itally-Stained Zebrafish Embryos Mark S. Cooper Leonard A. D'Amico and Clarissa A. Henry. Confocal Imaging of Living Cells in Intact Embryos Paul M. Kulesa and Scott E. Fraser. Live Confocal Analysis with Fluorescently Labeled Proteins Helen Francis-Lang Jonathan Minden William Sullivan and Karen Oegema. Live Imaging with Green Fluorescent Protein Jim Haseloff Emma-Louise Dormand and Andrea H. Brand. Fluorescent Calcium Indicators: Subcellular Behavior and Use in Confocal Imaging Donald M. O'Malley Barry J. Burbach and Paul R. Adams. Intracellular pH and pCa Measurement Michal Opas and Ewa Dziak. Measuring Dynamic Cell Volume In Situ By Confocal Microscopy Rachel J. Errington and Nick S. White. Imaging Thick Tissues with Confocal Microscopy Delphine Imbert Janet Hoogstraate Emmaline Marttin and Chris Cullander. Measurement in the Confocal Microscope Guy Cox. Presentation of Confocal Images Georg Halder and Stephen Paddock. The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides Gabriel G. Martins Alan T. Stonebraker and Robert G. Summers. Traditional Text-Based Databases and Image Gallery Databases Harvey Karten. Morphing Confocal Images and Producing Digital MoviesEric Hazen.Index.

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