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In Situ Hybridization Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 326

Edited By: Ian A Darby and Tim D Hewitson

269 pages, photos, diagrams

Humana Press

Hardback | Dec 2006 | Edition: 3 | #163646 | ISBN: 1588294021
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £115.50 $141/€129 approx

About this book

This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

From Reviews of the First Edition by Andy Choo... "...a very helpful manual...essential for any laboratory that is using or wants to use the in situ hybridization technique in their repertoire." - - Molecular Biotechnology " ...a good book for anyone wishing to exploit all that in situ hybridization has to offer." - - Biopharm Manufacturing Reviews for Second Edition This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky technique The text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both. - Heredity


Treatment of Tissue Sections for In Situ Hybridization; Gregory H. Tesch, Hui Y. Lan, and David J. Nikolic-Paterson; Preparation of Template DNA and Labeling Techniques; Peter J. Roche; In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods; Jan A. Darby, Teresa Bisucci, Alexis Desmouliere, and Tim D. Hewitson; Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization; Ernst J. M. Speel, Anton H. N. Hopman, and Paul Komminoth; Expression Analysis of Murine Genes Using In Situ Hybridization With Radioactive and Nonradioactively Labeled RNA Probes; Anne Chotteau-Lelievre, Pascal Dolle, and Francoise Gofflot; Nonradioactive In Situ Hybridization on Frozen Sections and Whole Mounts; Julia Asp, Alexandra Abramsson, and Christer Betsholtz.; In Situ Hybridization of Whole-Mount Embryos; Murray Hargrave, Josephine Bowles, and Peter Koopman; In Situ Detection of Epstein - Barr Virus and Phenotype Determination of EBV-Infected Cells; Gerald Niedobitek and Hermann Herbst; PNA - In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent Detection of Cellular and Viral Proteins; Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda; Electron Microscopy and In Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum; Andrzej Loesch and Rainer Glass; In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections; Neil C. Owens, F. Martin Hess, and Emilio Badoer; An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells; Changsun Choi, Laurie G. Hudson, Pierre Savagner, and Donna F. Kusewitt; Identification of Transplanted Human Cells in Animal Tissues; Daniel Benten, Kang Cheng, and Sanjeev Gupta; In Situ Hybridization to Plant Tissues and Chromosomes; Andreas Houben, Sharon J. Orford, and Jeremy N. Timmis; Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA; Tim D. Hewitson, Kristen J. Kelynack, and Ian A. Darby; Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA; Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby; The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue. Sections; Malcolm D. Smith, Michael Ahern, and Mark Coleman; Semiquantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Populations; Paul D. Storer and Tracey DeLucia; Quantitative In Situ Hybridization of Tissue Microarrays; Adrian M. Jubb, Thinh Q. Pham, Gretchen D. Frantz, Franklin V. Peale, Jr., and Kenneth J. Hillan; Index.

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