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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Antibody Engineering: A Practical Approach

Handbook / Manual
Series: Practical Approach Series Volume: 169
Edited By: John McCafferty, Hennie Hoogenboom and Dave Chiswell
Publisher: IRL Press
Antibody Engineering: A Practical Approach
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  • Antibody Engineering: A Practical Approach ISBN: 9780199635924 Paperback Jun 1996 Usually dispatched within 2-3 weeks
    £130.00
    #83946
  • Antibody Engineering: A Practical Approach ISBN: 9780199635931 Hardback Dec 1996 Out of Print #83947
Selected version: £130.00
About this book Contents Customer reviews Related titles

About this book

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities.

Contents

1. Construction and use of antibody gene repertoires; 2. Affinity maturation of antibodies using phage display; 3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs; 4. Measuring antibody affinities in solution; 5. Measuring antibody affinities using Bio-sensors; 6. Analysis of human antibody sequences; 7. Rodent to human antibodies by CDR grafting; 8. Rodent to human antibodies by phage display; 9. Choosing and manipulating effector functions; 10. Secretion and purification of antibody fragments expressed in bacteria; 11. Production of single chain Fv monomers and multimers; 12. Expression of immunoglobulin genes in mammalian cells; 13. In vitro linkage of antibody fragments into dimeric and multimeric forms

Customer Reviews

Handbook / Manual
Series: Practical Approach Series Volume: 169
Edited By: John McCafferty, Hennie Hoogenboom and Dave Chiswell
Publisher: IRL Press
Media reviews
This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions.--Doody's Health Sciences Book Review Home Page
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