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Antibody Engineering: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 169

Edited By: John McCafferty, Hennie Hoogenboom and Dave Chiswell

IRL Press

Paperback | Jun 1996 | #83946 | ISBN: 0199635927
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £130.00 $172/€148 approx
Hardback | Dec 1996 | #83947 | ISBN: 0199635935
Out of Print Details

About this book

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities.

This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions.--Doody's Health Sciences Book Review Home Page


1. Construction and use of antibody gene repertoires; 2. Affinity maturation of antibodies using phage display; 3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs; 4. Measuring antibody affinities in solution; 5. Measuring antibody affinities using Bio-sensors; 6. Analysis of human antibody sequences; 7. Rodent to human antibodies by CDR grafting; 8. Rodent to human antibodies by phage display; 9. Choosing and manipulating effector functions; 10. Secretion and purification of antibody fragments expressed in bacteria; 11. Production of single chain Fv monomers and multimers; 12. Expression of immunoglobulin genes in mammalian cells; 13. In vitro linkage of antibody fragments into dimeric and multimeric forms

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