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About this book
Provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system, including the genetic resources available and approaches for the identification, isolation and characterisation of genes to aid in understanding complex plant processes.
Contents
Growth, maintenance and use of Arabidopsis genetic resources; Preservation and handling of stock centre clones; Genetic mapping using recombinant inbred lines; ARABIDOPSIS MUTANT CHARACTERISATION: MICROSCOPY, MAPPING AND GENE EXPRESSION ANALYSIS; Classical and molecular cytogenetics of Arabidopsis; Tissue culture, transformation and transient gene expression in Arabidopsis; Transposon and T-DNA mutagenesis; Map based cloning in Arabidopsis; YACs, BACs, cosmids and nucleotide sequences; Web-based bioinformatic tools for Arabidopsis researchers
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