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Through the study and application of bioluminescence, scientists have painstakingly harnessed a powerful tool that enables us to seek a deeper understanding of the complex mechanisms underpinning so many vital biological systems. In this fully revised and updated second edition of Bioluminescence: Methods and Protocols, expert researchers contribute a readable and utilitarian compilation of the newest and most innovative techniques that have emerged in this rapidly expanding and progressively diverse field including methods to assess cell trafficking, protein-protein interactions, intracellular signaling, and apoptosis.
Also opening up the possibility to visualize and quantify biological mechanisms in real-time and in in vivo settings, the volume also describes the in vivo study of bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials, equipment, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bioluminescence: Methods and Protocols, Second Edition provides protocols that are detailed enough to be followed and adapted by scientific teams who have no previous expertise in bioluminescence in order to foster the potentially numerous breakthroughs and new applications from basic to applied science and medicine that must continue to be developed.
1. Luminescent Probes and Visualization of Bioluminescence Elisa Michelini, Luca Cevenini, Laura Mezzanotte, and Aldo Roda 2. Validation of Bioluminescent Imaging Techniques John Virostko and E. Duco Jansen 3. Assessment of Extracellular ATP Concentrations Lucia Seminario-Vidal, Eduardo R. Lazarowski, and Seiko F. Okada 4. High Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden Angelina Contero, Edmond Richer, Ana Gondim, and Ralph P. Mason 5. Fluorescence Imaging of Tumors with "Smart" pH-Activatable Targeted Probes Daisuke Asanuma, Hisataka Kobayashi, Tetsuo Nagano, and Yasuteru Urano 6. Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots Byron Ballou, Lauren A. Ernst, Susan Andreko, James A. J. Fitzpatrick, B. Christoffer Lagerholm, Alan S. Waggoner, and Marcel P. Bruchez 7. Bioluminescent Imaging of Transplanted Islets Xiaojuan Chen and Dixon B. Kaufman 8. Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cell Survival, Proliferation, and Fate Kitchener D. Wilson, Mei Huang, and Joseph C. Wu 9. Detection of Apoptosis Using Cyclic Luciferase in Living Mammals Akira Kanno, Yoshio Umezawa, and Takeaki Ozawa 10. Non-Invasive Bioluminescent Imaging of Infections Javier S. Burgos 11. Real-Time Bioluminescence Imaging of Viral Pathogenesis Kathryn E. Luker and Gary D. Luker 12. Bioluminescent Monitoring of in vivo Colonization and Clearance Dynamics by Light-Emitting Bacteria Siouxsie Wiles, Brian D. Robertson, Gad Frankel, and Angela Kerton 13. Quantitative In Vivo Imaging of Non-Viral Mediated Gene Expression and RNAi Mediated Knockdown Garrett R. Rettig and Kevin G. Rice 14. Analysis of Protein-Protein Interactions Using Bioluminescence Resonance Energy Transfer Kevin D.G. Pfleger 15. Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay Akira Kanno, Takeaki Ozawa, and Yoshio Umezawa 16. Bioluminescence Analysis of Smad-Dependent TGF-b Signaling in Live Mice Jian Luo and Tony Wyss-Coray 17. Bioluminescence Imaging of Calcium Oscillations Inside Intracellular Organelles Carlos Villalobos, Maria Teresa Alonso, and Javier Garcia-Sancho 18. Novel Tools for Use in Bioluminescence Resonance Energy Transfer (BRET) Assays Melanie Robitaille, Isabelle Heroux, Alessandra Baragli, and Terence E. Hebert 19. PIN-G Reporter for Imaging and Defining Trafficking Signals in Membrane Proteins Lynn Mckeown, Vicky C. Jones, and Owen T. Jones 20. Imaging ss-Galactosidase Activity in vivo Using Sequential Reporter-Enzyme Luminescence Georges von Degenfeld, Tom S. Wehrman, and Helen M. Blau
From the reviews of the second edition: "This is a compilation of laboratory protocols dealing with diverse applications of bioluminescence in the biological sciences. ! The book is written primarily for scientists working in the field of biomedical sciences. ! It also provides a number of updated protocols and examples for practicing investigators interested in using bioluminescence in their research. ! This second edition should prove to be a valuable companion to all researchers interested in bioluminescence." (Ruben Mestril, Doody's Review Service, December, 2009)