Embryo Culture Methods and Protocols

About this book

Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies.

Contents

1. Historical Background of Gamete and Embryo Culture Jacques Cohen and Don Rieger
2. Utility of Animal Models for Human Embryo Culture Development - Rodents Gary D. Smith
3. Utility of Animal Models for Human Embryo Culture Development-Domestic Species Rebecca L. Krisher
4. Utility of Animal Models for Human Embryo Culture: Non-Human Primates Calvin Simerly and Gerald Schatten
5. Media Composition: Salts and Osmolality Jay M. Baltz
6. Media Composition: Energy Sources and Metabolism Deirdre Zander-Fox and Michelle Lane
7. Media Composition - Amino Acids and Cellular Homeostasis Franchesca D. Houghton
8. Media Composition: Macromolecules and Embryo Growth Marius Meintjes
9. Media Composition- Antioxidants/Chelators and Cellular Function Catherine M.H. Combelles and Margo L. Hennet
10. Media Composition: pH and Buffers Jason E. Swain
11. Media Composition, Growth Factors Aparna Hegde
12. Culture Systems - Single Step Ronit Machtinger, Catherine Racowsky
13. Culture Systems - Sequential Patrick Quinn
14. Embryo Coculture Yves J R Menezo , Edouard Servy, Anna Veiga, Andre Hazout , Kay Elder
15. Low Oxygen Culture Borut Kovacic
16. Culture Methods - Embryo density Michael L. Reed
17. Culture Medium - Air Quality Theodore Thomas
18. Mineral Oil Overlay and Embryo Culture Dean E. Morbeck and Phoebe M. Hughes
19. Physiological and Environmental Factors That Can Affect the Outcome of Human ART Don Rieger
20. Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics) Andre Monteiro da Rocha and Gary D. Smith
21. Human Embryo Culture Media Comparisons Thomas B. Pool, John Schoolfield and David Han
22. Embryo Culture and Monozygotic Twinning Amy E. Sparks
23. Embryo Culture and Epigenetics Brenna A. Market Velker, Michelle M. Denomme and Mellissa R. W Mann

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