Protein Acetylation Methods and Protocols

About this book

This Methods in Molecular Biology book covers techniques for identifying protein acetylation, column- and gel electrophoresis-based approaches, computational predictionand biological response to protein acetylation. Includes protocols and troubleshooting tips.

Contents

1. Validation of Protein Acetylation by Mass Spectrometry Barry M. Zee and Benjamin A. Garcia
2. Application of the CIRAD Mass Spectrometry Apprach for Lysine Acetylation Site Discovery Caroline A Evans, Saw Yen Ow, Duncan L Smith, Bernard M Corfe, and Phillip C Wright
3. Application of the MIDAS Approach for Analysis of Lysine Acetylation SitesCaroline A Evans, John R Griffiths, Richard D Unwin, Anthony D Whetton, Bernard M Corfe
4. Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein AcetylationsBernard M Corfe, Josephine Kilner, Joanna Chowdry, Roderick SP Benson, Gareth J Griffiths, Caroline A Evans
5. Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin Xumin Zhang and Peter Hojrup
6. Identification and Analysis of O-acetylated Sialoglycoproteins Chandan Mandal and Chitra Mandal
7. HPLC-based Quantification of in vitro N-terminal Acetylation Rune H. Evjenth, Petra Van Damme, Kris Gevaert, Thomas Arnesen
8. Separation and Purification of Multiply Acetylated Proteins using Cation-exchange Chromatography Romeo Papazyan and Sean D. Taverna
9. In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal Acetylation Petra Van Damme, Thomas Arnesen, Bart Ruttens, Kris Gevaert
10. Computational Prediction of Lysine Acetylation Proteome-wide Amrita Basu
11. Generation and Characterization of Pan-specific Anti-acetyllysine AntibodyDr. Wei Xu and Dr. Shimin Zhao
12. Using Functional Proteome Microarrarys to Study Protein Lysine Acetylation Jin-Ying Lu, Yu-yi Lin, Jef D. Boeke, and Heng Zhu
13. Quantitation of Nucleosome Acteylation and other Histone Post-Translational Using Microscale NU-ELISA Bo Dai, Charles Giardina, Theodore P. Rasmussen
14. Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core John C. Shimko, Cecil J. Howard, Michael G. Poirier, and Jennifer J. Ottesen
15. Production of Amino-terminally Acetylated Recombinant Proteins in E. coli Matthew Johnson, Michael A. Geeves and Daniel P. Mulvihill
16. Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical Proteomics Markus Grammel and Howard C. Hang
17. Non-radioactive in-vitro Assays for Histone Deacetylases Alexander-Thomas Hauser, Julia M. Gajer (nee Wagner), and Manfred Jung
18. The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes Tielong Gao, Chao Yang, Yujun George Zheng
19. Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators Mohammed Arif, Dhanasekaran Karthigeyan, Soumik Siddhanta, G.V. Pavan Kumar, Chandrabhas Narayana, and Tapas K. Kundu

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