Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein. Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given transcription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-protein interactions, and posttranslational modification. Part III explores how gene targeting techniques in mice can provide insight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediated alterations in gene expression in mammalian systems.
Part I. Gene Expression Control at the mRNA Level. Overview. Messenger RNA Accumulation. Transcript Profiling. Transcriptional Regulation of Gene Expression. Posttranscriptional Processing of Messenger RNA. Part II. Regulation of Protein Levels and Transcription Factor Function. Overview. Protein Synthesis and Turnover. Proteomics. Determination of Protein-Protein Interactions and the Motifs That Mediate Them. Posttranslational Modifications. Dissection of Signaling Pathways. Part III. Use of Transgenic and Knockout Mice to Study Gene Regulation. Overview. Production of Transgenic Animals. Knockout Animals. Isolation of Cells and Cell Lines From Transgenic and Knockout Animals. Index.
From the reviews: "The book is aimed at graduate students, filling a gap between more general text books ! and laboratory manuals (e.g. the Current Protocols series). ! has many clear illustrations and these are also available in an accompanying CD. Helpful notes and key references are given in the margins throughout the text. ! overall 'Regulation of Gene Expression: Molecular Mechanisms' would certainly be of use to life sciences undergraduates, it would also be a useful reference work for a Molecular Toxicology laboratory." (James Sidaway, British Toxicology Society Newsletter, Summer, 2007)