Differential Display

About this book

One of the most challenging tasks facing the modern biological research laboratory is to make sense of the enormous amount of data being generated by various genome projects currently underway, and especially the human genome project. Understanding the ways in which genes are differentially expressed in various tissues and cell types, throughout ontogenetic development and in pathological processes, will go a long way towards understanding the function of all these 'new' genes and their protein products. Differential Display explains in detail how to perform the technique of RT-PCR Differential Display in various kinds of experimental biological systems. It also examines this technique in the context of other methods of studying differential gene expression such as subtractive hybridisation and the use of high-density gene microarrays combined with hybridisation techniques and automatic image analysis.

Contents

An introduction to differential display and related techniques; Recent advances in fluorescent differential display; Practical aspects of the experimental design for differential display of transcripts obtained from complex tissues; RAP-array : expression profiling using reduced complexity probes for cDNA arrays; The use of RT-PCR differential display in single-celled organisms and plant tissues; A modified approach for the efficient display of 3' - end restriction fragments of cDNAs; Cloning of differentially expressed brain cDNAs; Use of suppression subtractive hybridization strategy to identify differential gene expression in brain ischemic tolerance; Studying gene expression profiles in specialized brain regions by microsage; Construction and applications of gene microarrays on nylon membranes; Subtracted differential display and antisense approaches to analyzing brain signalling; Differential display analysis of memory-associated genes

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