DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA and by providing a massive parallel platform for data gathering represent a fundamental technical advance in biomedical research. Such biochips enable the use of advanced fabrication, detection, and data mining technologies that allow data gathering at an unprecedented rate. The first chapter is an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips. The next two chapters describe the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. Chapter 4 describes assay optimization for enzymatic assays and chapter 5 antisense scanning arrays. This is followed by a chapter on the manufacture of molecular arrays using ink-jet printing technology. Chapter 7 describes gene expression analysis from cDNA microarrays and then chapter 8 covers the use of expression data in bioinformatics. Chapter 9 describes the use of active microelectronic arrays for DNA hybridization analysis and the last chapter details the use of microarray technology in pharmacogenomics. This Practical Approach book is a comprehensive overview of the new and expanding field of DNA microarray technology and will be invaluable to any researcher interested in the use of biochips.
"DNA Microarrays (biochips) are small glass chips embedded with ordered rows of DNA which allow data to be gathered quickly. This volume provides a guide to this new technology, addressing the use of confocal scanning in microarray detection, techniques for cloning and screening of differentially expressed genes, the optimization of enzymatic assays using oligonucleotide arrays, antisense oligonucleotide scanning arrays, the production of molecular arrays using ink-jet printing technology, gene expression analysis via cDNA microarrays, the use of expression data in bioinformatics, active microelectronic arrays for DNA hybridization analysis, and the applications of microarray technology in pharmacogenomics. In each article, methodological details and current applications are described. Contributors primarily consist of biochemists and researchers in pharmaceutical companies in the US, Switzerland, and the UK."--SciTech Book News
There are currently no reviews for this book. Be the first to review this book!