Immobilized Biomolecules in Analysis: A Practical Approach

About this book

Biomolecules and cells are critical components of biosensors and biomaterials, but in order to function in an artificial environment, they must be immobilized in a manner that does not affect their interaction with target analytes. Biosensors demonstrate that we can harness the incredible functions of living molecules and cells for our own purposes and are therefore at the forefront of technology. Moreover the applications of immobilized biomolecules and cells are expected to expand far beyond biosensor applications and indeed are already used for pharmaceutical production and testing. Biomaterials will become increasing common as they are being developed into toxic filters, artificial organs, and even silicon chips. This book provides a selection of methods for the immobilization of biomolecules and cells on a variety of surface with different geometries and chemistries so that they retain their function and guidelines on which method to use. Also included are the analytical techniques to measure the functionality of immobilized biomolecules. All the protocols have been tried and validated by the authors. Immobilized Biomolecules in Analysis: A Practical Approach is an invaluable guide to all researchers in the fields of biosensors and biomaterials. Research in biosensors is carried out in a wide variety of fields including biochemistry, chemistry, engineering, laboratory medicine, environmental and defence research. The protocols are written so that an extensive prior knowledge of biochemistry is not required to use them.

Contents

Preface; 1. Silane-modified surfaces for biomaterial immobilization; 2. Avidin-biotin immobilization systems; 3. Antibodies as immobilization reagents; 4. Bioanalytical applications of self-assembled monolayers; 5. Protein adsorption: friend or foe?; 6. Micropatterning cell adhesiveness; 7. Sol-gel matrices for protein entrapment; 8. Immobilization of 'smart' polymer-protein conjugates; 9. Kinetic analysis of the interaction between an analyte in solution and an immobilized protein; 10. Spectroscopic characterization of immobilized proteins; 11. Immobilization using electrogenerated polymers; Appendix; Index

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