Neurochemistry: A Practical Approach

About this book

Neurochemistry is the study of the cellular and subcellular components of the nervous system and the molecules found within it. The nervous system is complex and neurochemistry is a diverse field in which there has been much interest recently, for example in the increased awareness of pathological brain conditions such as Alzheimer's disease, depression, and mania. A wide range of techniques are available to the neurochemist and this volume contains the core elements of neurochemistry that should be useful to both first time researchers and established investigators in all fields of neuroscience.

Contents

1. Isolation of synaptosomes, growth cones and their subcellular components; 2. Proteins of the neuronal cytoplasmic and membrane-associated cytoskeleton; 3. Immunocytochemical techniques for the study of the nervous system; 4. In situ hybridization approaches to the study of the nervous sytem; 5. Investigation of mitochondrial defects in brain and skeletal muscle; 6. Measurement of neurotransmitters and their associated second messengers in vivo; 7. Protein phosphorylation and dephosphorylation in the nervous system; 8. Receptor-linked phosphoinositide metabolism; 9. Neurotransmitter transporters: purification, reconstitution, and assay of the brain GABA transporter

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