Plant Embryogenesis

About this book

The advent of the postgenome era has brought a remarkable evolution in modern plant embryology. In Plant Embryogenesis, the authors focus on the common developmental process and compile novel tools and methods designed specifically for studying plant embryogenesis. In each chapter, detailed outlines of model embryonic systems are provided, as well as cutting-edge cellular, genetic and molecular mechanisms. This important book gives insight into the methods used in studying the transformation of phylogenetically-conserved embryonic patterns into flora's astounding diversity of growth forms today. Invaluable to those new to the field and inspiring to full-fledged plant embryologists, Plant Embryogenesis provides today's most comprehensive platform to the understanding of plant embryo development.

Contents

Arabidopsis embryogenesis.- Maize embryogenesis.- Spruce embryogenesis.- In vitro fertilization with isolated higher plant gametes.- In vitro culture of Arabidopsis embryos.- Culture and time-lapse tracking of barley microspore-derived embryos.- Isolation of embryo-specific mutants in Arabidopsis: Plant transformation.- Isolation of embryo-specific mutants in Arabidopsis: Genetic and phenotypic analysis.- Laser-capture microdissection to study global transcriptional changes during plant embryogenesis.- Promoter trapping system to study embryogenesis.- Visualization of auxin gradients in embryogenesis.- Intercellular trafficking of macromolecules during embryogenesis.- Immunolocalisation of proteins in somatic embryos: applications for studies on the cytoskeleton.- Detection of programmed cell death in plant embryos.

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