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A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
A Historical Perspective of EGFR and Related Systems; Francis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera R. Pete, Deepti Chaturvedi, Paul J. Bertics and Tarun B. Patel; Purification and Assay of Kinase-Active EGF Receptor from Mammalian Cells by Immunoaffinity Chromatography; Gregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham and Paul J. Bertics; Soluble EGF Receptor (sEGFR) Acrydinium-Linked Immunosorbent Assay (ALISA); Jacqueline Laftky, Andre T. Baron and Nita Maihle; Analysis of EGF Receptor and Interactions with the Alpha Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs; Deepti Chaturvedi, Edwin Francis, Hui Sun and Tarun B. Patel; Regulator of EGF Signalling: Sprouty; Esther S. M. Wong and Graeme R. Guy; Dissecting the EGF Signal Transactivation Pathway; Oliver Fischer, Stephan Hart and Axel Ullrich; A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the EGF Receptor; Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi and Carl Blobel; In Vitro and in Vivo Assays of Mono-Ubiquitination of Receptor Tyrosine Kinases; Manachem Katz, Yaron Mosseson, and Yosef Yarden; Assays to Monitor Degradation of the EGF Receptor; Mirko H. H. Schmidt and Ivan Dikic; Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members; Adriano Marchese; Epithelial Cell Migration in Response to EGF; Reema Zeineldin and Laurie Hudson; Motility Signaled from EGF Receptors and Related Systems; Alan Wells, Brian Harms, Akihiro Iwabu, Lily Koo, Kirsty Smith, Linda Griffiths and Doulas Lauffenburger; Methods for Determining the Proliferation of Cells in Response to EGFR Ligands; Paul J. Bertics, Gregory J. Wiepz, Francis Edwin and Tarun B. Patel; Clinical Advancement of EGFR Inhibitors in Cancer Therapy; Prakash Chinnaiyan and Paul M. Harari.
From the reviews: "The role of epidermal growth factor (EGF) in both normal development and malignancy has been elucidated gradually over the past half century ! . this book could well prove a great source of help and inspiration to anyone trying to develop kits to simplify and streamline the analysis of the EGF system. ! would also be a good introduction to EGF history, physiology and possible future developments, so it could be worth looking out for in the library ! ." (D. J. Cook, British Journal of Biomedical Science, Vol. 63 (2), 2006)