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This volume covers the procedures needed for the identification of specific genes and the study of their structure and cellular expression.
1. Strategies for research in molecular biology; 2. Construction of genomic libraries in lambda and cosmic vectors; 3. Construction of a cDNA library; 4. Nucleic acid labelling and detection; 5. Immobilization of nucleic acids and hybridization analysis; 6. DNA sequencing; 7. The polymerase chain reaction; 8. Protein expression in Escherichia coli; 9. Transcript mapping; 1. HEALTH HAZARDS AND SAFETY PROCEDURES; 2. Equipment for molecular biology research; 3. Important Escherichia coli strains; 4. Recipes and general procedures; 5. Restriction endonucleases; 6. DNA and RNA modification enzymes; 7. List of suppliers' addresses
"Practical information for working researchers in the life sciences interested in learning the basic principles of gene cloning and recombinant DNA techniques. The contributors provide the background material novices need to understand how a technique works, what it achieves, and how to make modifications to suit their own requirements, as well as advice on more advanced techniques. Vol. 2 describes procedures for isolating and studying individual genes, preparation and screening of libraries, polymerase chain reactions, DNA sequencing, and studying gene expression. The latest edition reflects the dramatic expansion in the range and sophistication of molecular biology techniques."--SciTech Book News