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About this book
Concentrates on the procedures for DNA and RNA manipulation: purification, electrophoresis, and the construction and cloning of recombinant molecules. Also includes a general introduction to molecular biology in the laboratory and a survey of cloning vectors for Escherichia Coli.
Getting Started in Molecular Biology; Microbiological Techniques for Molecular Biology: bacteria and phages; Purification of DNA; Purification of RNA; Nucleic Acid Electrophoresis in Agarose Gels; Recovery of DNA from Electrophoresis Gels; Construction of Recombinant DNA Molecules; Generation and Identification of Recombinant Clones; Survey of Cloning Vectors for Escherichia coli; A1: Important Escherichia coli Strains; A2: Recipes and General Procedures; A3: Restriction Endonucleases