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Immobilization of Enzymes and Cells

Handbook / Manual

Series: Methods in Biotechnology

Edited By: Jose M Guisan

449 pages, Line & b/w illus

Humana Press

Hardback | Dec 2006 | Edition: 2 | #163736 | ISBN: 1588292908
Availability: Usually dispatched within 1-2 weeks Details
NHBS Price: £139.99 $183/€159 approx

About this book

The second edition of Immobilization of Enzymes and Cells serves as an update as well as a compliment to the first edition. This volume now includes simple protocols for the immobilization of enzymes and cells that could be useful for application at industrial scale, novel protocols for immobilization in the future, and new chemical reactors able to overcome the limitations of a number of immobilized derivatives.

Many well-known authors from research laboratories worldwide have contributed to this successful volume...this book provides a basic reference tool for all those who will need to immobilize biological materials in their work - not only for the beginner in the field, but also for experienced researchers. - Applied Biochemistry and Biotechnology


Immobilization of Enzymes and Cells: Some Practical Considerations Gordon F. Bickerstaff. Immobilization of Enzymes by Selective Adsorption on Biotinylaminopropyl Celite or Glass Harold E. Swaisgood Xiaolin L. Huang and Marie K. Walsh. Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass Violeta G. Janolino and Harold E. Swaisgood. Enzyme Immobilization on Nylon Pedro Lozano and Jos' L. Iborra. Visual Assessment of Enzyme Immobilization Arturo Manj##n and J##se L. Iborra. Immobilization in Carrageenans J##se L. Iborra Arturo Manj##n and Manuel C novas. Entrapment in Calcium Alginate Jane E. Fraser and Gordon F. Bickerstaff. Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports Maria Cantarella Francesco Alfani Laura Cantarella and Alberto Gallifuoco. Activation of Rayon/Polyester Cloth for Protein Immobilization Hiroshi Yamazaki and C. Scott Boyd. Immobilization of Enzymes on Microelectrodes Using Chemical Crosslinking Milena Koudelka-Hep Nico F. de Rooij and David J. Strike. Photolithographic Patterning of Enzyme Membranes for the Modification of Microelectrodes Milena Koudelka-Hep Nico F. de Rooij and David J. Strike. Electrochemical-Based Immobilization of Enzymes David J. Strike Nico F. de Rooij and Milena Koudelka-Hep. Immobilization of Enzymes on Thermo-Responsive Polymers Kazuhiro Hoshino Setuko Akakabe Shoichi Morohashi and Toshisuke Sasakura. Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix Robert Carpentier. Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization Guy Fortier Nicole Demers Jacques-Jean Fran+ois Jean-Charles Gayet and Edith M. D'Urso. Poly(Carbamoyl Sulfonate) Hydrogels Andreas Muscat and Klaus-Dieter Vorlop. Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles Gilbert Bardeletti. Immobilization of Enzymes and Proteins on Red Blood Cells Laura Chiarantini and Mauro Magnani. Cellulose Paper Support for Immobilization Marion Paterson and John F. Kennedy. Immobilization of Cells Using Electrostatic Droplet Generation Mattheus F. A. Goosen Eltag S. E. Mahmud Abdullah S. Al-Ghafri Hamad A. Al-Hajri Yousuf S. Al-Sinani and Branko Bugarski. Hepatocyte Immobilization in Agarose and Functional Integrity Testing Hassan Farghali and Sixtus Hynie. Immobilized Hepatocytes in Xenobiotic Biotransformation Studies Sixtus Hynie Ludmila Kamenikova and Hassan Farghali. Immobilization of Liposomes and Proteoliposomes in Gel Beads Eggert Brekkan Qing Yang Gerhard Viel and Per Lundahl. Cell Immobilization with Phosphorylated Polyvinyl Alcohol (PVA) Gel Kuo-Cheng Chen and Jer-Yiing Houng. Covalent Immobilization of Enzymes to Graphitic Particles Marco F. Cardosi. Enzyme Immobilization Using Chitosan-Xanthan Complexes Severian Dumitriu Pierre Vidal and Esteban Chornet. Calcium Alginate Film Formed on a Stainless Steel Mesh Harold E. Swaisgood and Flavia M. L. Passos. Preparation of Immobilized Subunits of a Multisubunit Enzyme Gordon F. Bickerstaff. Characterization of Enzyme Activity Protein Content and Thiol Groups in Immobilized Enzymes Gordon F. Bickerstaff. Immobilization of Enzymes Acting on Macromolecular Substrates: Reduction of Steric Problems Jos' M. Guis n Guadalupe Penzol Pilar Armisen Agatha Bastida Rosa M. Blanco Roberto Fern ndez-Lafuente and Eduardo Garc##a-Junceda. Immobilization of Enzymes on Glyoxyl-Agarose: Strategies for Enzyme Stabilization by Multipoint Attachment Jos' M. Guis n Agatha Bastida Rosa M. Blanco Roberto Fern ndez-Lafuente and Eduardo Garc##a-Junceda. Stabilization of Immobilized Enzymes by Chemical Modification with Polyfunctional Macromolecules Jos' M. Guis n Ver##nica Rodriguez Cristina M. Rosell Gloria Soler Agatha Bastida Rosa M. Blanco Roberto Fern ndez-Lafuente and Eduardo Garc##a-Junceda. Covalent Immobilization of Enzymes Using Commercially Available CDI-Activated Agarose George J. Piazza and Marjorie B. Medina. Immobilization of Cells in Polyelectrolyte Complexes Johanna Mansfeld and Horst Dautzenberg. Coimmobilization of Enzymes and Cells Johanna Mansfeld and Horst Dautzenberg. Adsorption of Lipase on Inorganic Supports Jos' V. Sinisterra. Immobilization of Enzymes on Inorganic Supports by Covalent Methods Jos' V. Sinisterra. Use of Divalent Metal Ions Chelated to Agarose Derivatives for Reversible Immobilization of Proteins Robert R. Beitle Jr. and Mohammad M. Ataai. Transition Metal Methods for Immobilization of Enzymes and Cells John F. Kennedy Joaquim M. Cabral Maria R. Kosseva and Marion Paterson. Index.

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