About this book
This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Treatment of Tissue Sections for In Situ Hybridization; Gregory H. Tesch, Hui Y. Lan, and David J. Nikolic-Paterson; Preparation of Template DNA and Labeling Techniques; Peter J. Roche; In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods; Jan A. Darby, Teresa Bisucci, Alexis Desmouliere, and Tim D. Hewitson; Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization; Ernst J. M. Speel, Anton H. N. Hopman, and Paul Komminoth; Expression Analysis of Murine Genes Using In Situ Hybridization With Radioactive and Nonradioactively Labeled RNA Probes; Anne Chotteau-Lelievre, Pascal Dolle, and Francoise Gofflot; Nonradioactive In Situ Hybridization on Frozen Sections and Whole Mounts; Julia Asp, Alexandra Abramsson, and Christer Betsholtz.; In Situ Hybridization of Whole-Mount Embryos; Murray Hargrave, Josephine Bowles, and Peter Koopman; In Situ Detection of Epstein - Barr Virus and Phenotype Determination of EBV-Infected Cells; Gerald Niedobitek and Hermann Herbst; PNA - In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent Detection of Cellular and Viral Proteins; Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda; Electron Microscopy and In Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum; Andrzej Loesch and Rainer Glass; In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections; Neil C. Owens, F. Martin Hess, and Emilio Badoer; An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells; Changsun Choi, Laurie G. Hudson, Pierre Savagner, and Donna F. Kusewitt; Identification of Transplanted Human Cells in Animal Tissues; Daniel Benten, Kang Cheng, and Sanjeev Gupta; In Situ Hybridization to Plant Tissues and Chromosomes; Andreas Houben, Sharon J. Orford, and Jeremy N. Timmis; Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA; Tim D. Hewitson, Kristen J. Kelynack, and Ian A. Darby; Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA; Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby; The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue. Sections; Malcolm D. Smith, Michael Ahern, and Mark Coleman; Semiquantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Populations; Paul D. Storer and Tracey DeLucia; Quantitative In Situ Hybridization of Tissue Microarrays; Adrian M. Jubb, Thinh Q. Pham, Gretchen D. Frantz, Franklin V. Peale, Jr., and Kenneth J. Hillan; Index.