Microfluidic Techniques Reviews and Protocols

About this book

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Contents

Overview of Advances in Microfluidics and Microfabrication; Shelley D. Minteer and Christine M. Moore; Part I. Microfabrication Methods; Introduction to Microfabrication Techniques; Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou; Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds; Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou; Fabrication of Microelectrodes Using the Lift-Off Technique; Benjamin Y. Park, Rabih Zaouk, and Marc J. Madou; Laser Ablation as a Fabrication Technique for Microfluidic Devices; Emanuel A. Waddell; Reversible, Room Temperature Bonding of Glass Devices for Microfluidics; Loranelle L. Lockyear; Part II. Biological Applications; Solid-Phase Extraction in Packed Beds on Glass Microdevices; Loranelle L. Lockyear; Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems; Jin-Woo Choi; Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: Hydrogel Immunoassay; Gloria Thomas, Emad M. El-Giar, Laurie E. Locascio, and Michael J. Tarlov; Polymerase Chain Reaction in Miniaturized Systems: Big Progress in Little Devices; Kalee D. Spitzack and Victor M. Ugaz; Polymerase Chain Reaction on Microchips; Maria C. Carles and Nikolaus J. Sucher; A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes; Xiaojun Zhao, David T. Pierce, and Yanfu Huan; Microfluidic Ethanol Biobatteries on a Microchip; Sheeley D. Minteer and Christine M. Moore; Microfluidic Chambers for Cell Migration and Neuroscience Research; Anne M. Taylor, Seog Woo Rhee, and Noo Li Jeon; Microtextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem Cells; Erik T. K. Peterson and Ian Papautsky; Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip; Xiujun (James) Li and Paul C. H. Li. Microscale Integrated Sperm Sorter; Yaokuang Chung, Xiaoyue Zhu, Wei Gu, Gary D. Smith, and Shuichi Takayama; Index.

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