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Provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts, which will help to develop better methods for disease control in plants and animals. Plant-Pathogen Interactions: Methods and Protocols reviews methods for engineering resistance to plant viruses, the utility of viral induced gene silencing and RNAi silencing, as well as advances in genomics and proteomics that have lead to new methods to identify genes and proteins. The protocols presented offer step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.
The Use of Protoplasts to Study Innate Immune Responses Ping He, Libo Shan, and Jen Sheen Marker Exchange Mutagenesis and Complementation Strategies for the Gram- negative Bacteria Xanthomonas oryzae pv. oryzae Sang-Won Lee and Pamela C. Ronald Whole Genome Analysis to Identify Type III Secreted Effectors Boris A. Vinatzer and Jean T. Greenberg In planta Expression of Oomycete and Fungal Genes Thirumala-Devi Kanneganti, Edgar Huitema, and Sophien Kamoun Use of Nipponbare BAC Clones for Physical Mapping of an R Gene Locus in Rice Jong-Seong Jeon and Pamela C. Ronald Agrobacterium-Mediated Transformation to Create and Insertion Library in Magnaporthe grisea Sara L. Tucker and Marc J. Orbach Identification of Components in Disease Resistance Signaling in Arabidopsis by Map-Based Cloning Yuelin Zhang, Jane Glazebrook, and Xin Li Yeast Two-hybrid Approaches to Dissecting the Plant Defense Response Mawsheng Chern, Todd Richter, and Pamela C. Ronald Use of Rolling Circle Amplification for Large-Scale Yeast Two-Hybrid Analyses Xiaodong Ding, Yan Zhang, and Wen-Yuan Song Preparative Denaturing Isoelectric Focussing for Enhancing Sensitivity of Proteomic Studies Antonio Serna-Sanz, Greg Rairdan, and Scott C. Peck Use of MPSS to Study Genes Expressed during the Plant Defense Response Blake C. Meyers, Christian D. Haudenschild, and Kalyan Vemaraju Use of Microarray Analysis to Dissect the Plant Defense Response Jane Glazebrook Use of RL-SAGE Analysis to Identify Novel Fungal and Plant Genes Involved in Host-Pathogen Interactions Malali Gowda, Venu Reddyvarichannarayappa, Yulin Jia, Eric Stahlberg, Vishal Pampanwar, Carol Soderlund, and Guo-Liang Wang Analysis of Gene Function in Rice Through Virus-Induced Gene Silencing Xin Shun Ding, C. Srinivasa Rao, and Richard S. Nelson Use of RNA Interference to Dissect Defense Signaling Pathways in Rice Chuansheng Mei, Xiangjun Zhou, and Yinong Yang Virus-Induced Gene Silencing in Plant Roots Isgouhi Kaloshian Methods for Engineering Resistance to Plant Viruses M. R. Sudarshana, G. Roy, and B.W. Falk Development of Genetically Engineered Resistant Papaya for Papaya ringspot virus in a Timely Manner: A Comprehensive and Successful Approach Savarni Tripathi, Jon Suzuki, and Dennis Gonsalves
From the reviews: "In a highly lucid manner, this book describes new developments in generic techniques and others which are exclusive to plant pathologists. As a result this book will be of interest to seasoned practioners in the field as much as new post-graduate students. ! The presentation of each section is exemplary, with a useful summary, a concise introduction to each section and a step-by-step protocol. ! this is a wide-ranging protocols book that will be useful to plant pathologists at all stages in their careers." (Dr Luis Mur, Molecular Biotechnology, 2007) "The book is divided into 18 chapters ! . this book is an excellent reference for people interested in validating or improving existing lab protocols. ! serve as a basic reference work for those entering into a new research area. ! Texts are clearly written and the tabular format makes it easier to adopt novel methods step-by-step. This makes the book also a valuable tool for people involved in teaching. All in all, I can recommend this book as a good value for the money." (Ulrich Schaffrath, Journal of Plant Physiology, Vol. 165, 2008) "In this volume, the editor has assembled a versatile collection of papers that focus on large-scale analyses of plant-pathogen interactions. ! With contributions from over 40 experts in their fields, this stepwise set of protocols ! will certainly be of interest to molecular plant-pathogen researchers." (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, 2007)