Protein NMR Techniques Methods and Protocols

About this book

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein NMR Techniques,Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.

Contents

1. A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins
2. Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis
3. Isotope Labeling in Insect Cells
4. Isotope Labeling in Mammalian Cells
5. Cell-free Protein Production for NMR Studies
6. Cell-free Membrane Protein Expression for Solid-state NMR
7. Expression and Purification of Src-family Kinases for Solution NMR Studies
8. NMR Studies of Large Protein Systems
9. Protein Dynamics by 15N Nuclear Magnetic Relaxation
10. Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel
11. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy
12. NMR Studies of Protein-RNA Interactions
13. Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies
14. NMR Studies of Protein-ligand Interactions
15. In-cell NMR Spectroscopy in Escherichia coli
16. Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state NMR
17. Solid-State NMR Spectroscopy of Protein Complexes
18. Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy
19. Assignment of Backbone Resonances in a Eukaryotic Protein Kinase - ERK2
20. Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility
21. Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy
22. Comprehensive Automation for NMR Structure Determination of Proteins
23. ARIA for Solution and Solid-state NMR Protein NMR Techniques
24. Determining Protein Dynamics from 15N Relaxation Data by using DYNAMICS

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