Books  Ecological & Biological Sciences  Animal & General Biology  Biochemistry & Molecular Biology 

Protein NMR Techniques: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 831

By: Alexander Shekhtman(Editor), David S Burz(Editor)

532 pages, 23 colour & 84 b/w illustrations, 11 tables

Humana Press

Hardback | Dec 2011 | Edition: 3 | #205447 | ISBN-13: 9781617794797
Availability: Usually dispatched within 1 week Details
NHBS Price: £149.99 $191/€167 approx

About this book

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein NMR Techniques,Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.


1. A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins
2. Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis
3. Isotope Labeling in Insect Cells
4. Isotope Labeling in Mammalian Cells
5. Cell-free Protein Production for NMR Studies
6. Cell-free Membrane Protein Expression for Solid-state NMR
7. Expression and Purification of Src-family Kinases for Solution NMR Studies
8. NMR Studies of Large Protein Systems
9. Protein Dynamics by 15N Nuclear Magnetic Relaxation
10. Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel
11. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy
12. NMR Studies of Protein-RNA Interactions
13. Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies
14. NMR Studies of Protein-ligand Interactions
15. In-cell NMR Spectroscopy in Escherichia coli
16. Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state NMR
17. Solid-State NMR Spectroscopy of Protein Complexes
18. Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy
19. Assignment of Backbone Resonances in a Eukaryotic Protein Kinase - ERK2
20. Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility
21. Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy
22. Comprehensive Automation for NMR Structure Determination of Proteins
23. ARIA for Solution and Solid-state NMR Protein NMR Techniques
24. Determining Protein Dynamics from 15N Relaxation Data by using DYNAMICS

Write a review

There are currently no reviews for this book. Be the first to review this book!

Bestsellers in this subject

RNA Biology

NHBS Price: £54.99 $70/€61 approx

Ancestral Sequence Reconstruction

NHBS Price: £102.50 $131/€114 approx

The Cyanobacteria

NHBS Price: £215.00 $274/€240 approx

Stress Biology of Cyanobacteria

NHBS Price: £51.99 $66/€58 approx

Cell Biology [International Student Version]

NHBS Price: £48.39 $62/€54 approx

VAT: GB 407 4846 44
NHBS Ltd is registered in England and Wales: 1875194