In this book expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers.
Additionally, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes.
Section A: Micropropagation and Chemical Analysis 1. Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives Kee Yoeup Paek, Hosakatte Niranjana Murthy, and Eun Joo Hahn 2. In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium, Assessment of Genetic Fidelity of Micropropagated Plants, and Simultaneous Quantization of Reserpine, Ajmaline and Ajmalicine M. K. Goel, S. Mehrotra, A. K. Kukreja, K. Shanker, and S.P.S. Khanuja 3. Medicinal Properties, in vitro Protocols and Secondary Metabolite Analyses of Scots Pine Hely Haggman, Anna Maria Pirttila, Karoliina Niemi, Tytti Sarjala, and Riitta Julkunen-Tiitto 4. Saussurea medusa Cell Suspension Cultures for Flavonoid Production Chun-Zhao Liu and Praveen K. Saxena 5. Micropropagation of Crataeva nurvala Shashi B. Babbar, Neetika Walia, and Amandeep Kaur 6. Bilberry In vitro Protocols and Analyses of Phenolic Compounds Laura Jaakola, Kaisu Riihinen, Hely Haggman, and Anja Hohtola 7. In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana S. Pillai Geetha, AV. Raghu, Gerald Martin, Satheesh George, and Indira Balachandran 8. In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R.Br. Ex Roemer & Schultes through Callus Culture Under Abiotic Stress Conditions Abdul Bakrudeen Ali Ahmed, Adhikarla Suryanarayana Rao, and Mandali Venkateswara Rao 9. Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis Suk Weon Kim and Myung Jin Oh 10. In vitro Culture and Secondary Metabolite Isolation in Bryophytes Aneta Sabovljevic, Marko Sabovljevic, and Nebojsa Jockovic 11. Micropropagation and In vitro Conservation of Vanilla (Vanilla planifolia Andrews.) Minoo Divakaran and K. Nirmal Babu 12. Protocol for In vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham Sushma Kaul, K.A. Suri, P. Dutt, M. Sambyal, A. Ahuja, and M.K. Kaul 13. Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria Ian B. Cole, Faisal T. Farooq, and Susan J. Murch 14. Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae) Elizabete Catapan, Fabio Netto Moreno, Marcio Luis Busi da Silva, Michel Fleith Otuki, Rivaldo Niero, Valdir Cechinel Filho, Rosendo Augusto Yunes, and Ana Maria Viana 15. In vitro Clonal Propagation of Asparagus racemosus, a High Value Medicinal Plant Sanjay Saxena and Nishritha Bopana 16. Micropropagation of Penthorum chinense through Axillary Bud Jun Yang and Zheng-song Peng Section B: Transgenic Approaches 17. Spontaneous Plant Regeneration and Production of Secondary Metabolites from Hairy Root Cultures of Centaurium erythraea Rafn Angelina Subotic, Sladana Jevremovic, Dragoljub Grubisic, Teodora Jankovic 18. Transgenic Hypericum perforatum G. Franklin, Margarida M. Oliveira, and Alberto C.P. Dias 19. Agrobacterium-Mediated Transformation of Ruta graveolens L. Karine Lievre, Thi Le Minh Tran, Sebastien Doerper, Alain Hehn, Paul Lacoste, Brigitte Thomasset, Frederic Bourgaud, and Eric Gontier 20. Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures Katarina Brunakova and Jan Kosuth 21. Catapol Production in Chinese Foxglove (Rehmannia glutinosa Libos.) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834 Sung Jin Hwang Section C: Molecular Markers and Microsatellites 22. Identification of Medicinal Plants and Plant Sequences: A Multiplexed MLPA Assay Roger A. Barthelson 23. Isolation of Microsatellites from Catharanthus roseus (L.) G. Don Using Enriched Libraries Bhumika Shokeen and Sabhyata Bhatia Section D: Biotransformation, Bioreactors, and Metabolomics 24. Production of Cinnamyl Glycosides in Compact Callus Aggregate Cultures of Rhodiola rosea Through Biotransformation of Cinnamyl Alcohol Zsuzsanna Gyorg and Anja Hohtola 25. Spearmint Plantlet Culture System as a Means to Study Secondary Metabolism Brent Tisserat, Mark Berhow, and Steven F. Vaughn 26. Bioreactor Production of Secondary Metabolites from Cell Cultures of Periwinkle and Sandalwood Jagan V. Valluri 27. Camptothecin Production by In Vitro Cultures and Plant Regeneration in Ophiorrhiza Species Takashi Asano, Hiroshi Sudo, Mami Yamazaki, and Kazuki Saito 28. Metabolomic Analysis of Ocotea odorifera Cell Cultures: A Model Protocol for Acquiring Metabolite Data Marcelo Maraschin, Paulo Fernando Dias, Enio Luiz Pedrotti, Hiliana Nunes Ferreira Morais, Ana Maria Viana, and Karl Vernon Wood 29. The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali Maziah Mahmood and Rosli Noormi Section E: Altered Gravity and Biotechnology 30. Plant Secondary Metabolism in Altered Gravity Lindsey K. Tuominen, Lanfang H. Levine, and Mary E. Musgrave 31. The Role of Biotechnology in the Production of the Anticancer Compound Podophyllotoxin Hemant Lata, Cassia S. Mizuno, and Rita M. Moraes
From the reviews: "This book ! covers a range of topics vital for the continued production of this essential but sometimes forgotten group of plants. ! The chapters are easy to read and the protocols are easy to follow. All the necessary information is within the chapter. ! In conclusion, I would recommend this book to researchers and students working in the area of plant tissue culture and biochemical analysis. This book would also be beneficial as a text book or laboratory manual in plant tissue culture courses." (Alison M. R. Ferrie, Annals of Botany, Vol. 105 (4), April, 2010) "This technical guide for plant research helps explains the processes Step by-step procedures describemethods of analyzing plant compounds, identify molecular markers to find them ! . It includes materials researchers need and common pitfalls they can avoid. ! It is also designed to help conserve germ plasma and cryopreservation of medicinal plant species that face extinction or adverse effects from climate change." (The American Herb Association, Vol. 25 (2), 2010)