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Given the number of exciting developments across the whole spectrum of receptor research in recent years, the editors have not restricted themselves to one particular approach or class of receptors. Thus the studies within range from G protein-coupled surface receptors, to the delivery of antisense DNA inside living cell systems. A distinguished team of contributors cover these diverse areas, identifying any difficulties likely to be encountered and appropriate steps to overcome them. Wherever appropriate, the theoretical basis of each topic is explained first so that the results emerging from the practical procedures can be fully understood. Anyone with an interest in receptor structure and function will find this book an invaluable resource.
1. Probing G protein-coupled receptors by fluorescence spectroscopy; 2. From receptor to endogenous ligand; 3. Point mutations and chimeric receptors in studies of ligand binding domains; 4. Determination of the subunit composition of native and cloned NMDA receptors; 5. Evaluating receptor stoichiometry by fluorescence resonance energy transfer (FRET); 6. Monitoring intracellular trafficking of receptors; 7. Monitoring the turnover kinetics of G protein-coupled receptors; 8. Real time receptor function in vitro: microphysiometry and fluorometric imaging plate reader (FLIPR); 9. Regulation of receptors and receptor-coupled ion channels by protein phosphorylation; 10. Using confocal imaging to measure changes in intracellular ions; 11. Monitoring nuclear receptor function; 12. In vivo gene transfer of antisense to receptors